ABS44
抗-MAPK 1/2抗体
from rabbit
别名:
ERK-1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 3, MNK1, p44-ERK1, p44-MAPK, ERK-2, ERT1, Extrac
产品名称
抗-MAPK 1/2抗体, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse, rat
species reactivity (predicted by homology)
canine (based on 94% sequence homology), bovine (based on 94% sequence homology)
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MAPK1(5594)
Analysis Note
对照
A431 细胞裂解液
A431 细胞裂解液
通过蛋白质印迹在A431细胞裂解物中进行了评估。
蛋白质印迹分析：0.5 µg/mL该抗体可在10 µg A431细胞裂解物中检测到MAPK 3/MAPK 1（ERK1/ERK2）。
蛋白质印迹分析：0.5 µg/mL该抗体可在10 µg A431细胞裂解物中检测到MAPK 3/MAPK 1（ERK1/ERK2）。
Application
使用经验证可用于免疫细胞化学、免疫沉淀和蛋白质印迹的抗MAPK 1/2抗体检测MAPK 3/MAPK 1（ERK1/ERK2）。
研究子类别
MAP激酶
MAP激酶
研究类别
信号传导
信号传导
蛋白质印迹分析：0.5 µg/mL代表性批次在BALB/3T3、A431和L6细胞裂解物中检测到MAPK 3/MAPK 1（ERK1/ERK2）。
蛋白质印迹（SNAP ID）分析：1-2 µg/mL代表性批次在BALB/3T3、A431和L6细胞裂解物中检测到MAPK 3/MAPK 1（ERK1/ERK2）。
免疫细胞化学分析：代表性批次的1：500稀释液在NIH/3T3，A431，HeLa和C2C12细胞中检测到MAPK 3/MAPK 1（ERK1/ERK2）。
蛋白质印迹（SNAP ID）分析：1-2 µg/mL代表性批次在BALB/3T3、A431和L6细胞裂解物中检测到MAPK 3/MAPK 1（ERK1/ERK2）。
免疫细胞化学分析：代表性批次的1：500稀释液在NIH/3T3，A431，HeLa和C2C12细胞中检测到MAPK 3/MAPK 1（ERK1/ERK2）。
Biochem/physiol Actions
该抗体可识别MAPK 3/MAPK 1（ERK1/ERK2）C端序列。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
丝裂原活化蛋白激酶 3 （EC 2.7.11.24；UniProt P21708；也称为 ERK-1、ERT2、细胞外信号调节激酶 1、胰岛素刺激的 MAP2 激酶、MAP 激酶 3、MAP 激酶同工型 p44、MAPK 3、微管相关蛋白 2 激酶、MNK1、p44-ERK1、p44-MAPK）由大鼠物种中的 Mapk3（也称为 Erk1、Prkm3）基因（基因 ID 50689）编码。ERK1（又称 MAPK 3）和 ERK2（又称 MAPK 1 或 MAPK 2）是 Ras-Raf-MEK-ERK 信号通路的相关丝氨酸/苏氨酸激酶。ERK1/2 在其保守的 Thr-Glu-Tyr（TEY）双重磷酸化基序上由 MEK1/2 催化的磷酸化而被激活，首先在 Tyr 残基（人/大鼠 ERK1 Tyr204/Tyr203，人/大鼠 ERK2 Tyr187/Tyr183）和然后在 Thr 残基（人/大鼠 ERK1 Thr202/Thr201，人/大鼠 ERK2 Thr185/Thr181）。ERK1/2 是脯氨酸引导的蛋白激酶，可优先催化数百种胞质和核底物（包括 Ets、Elk 和 c-Fos 等转录因子）中 Pro-Xxx-Ser/Thr-Pro 序列基序的磷酸化。除了此基本结构要求外，许多 ERK1/2 底物还具有 D-停泊位点和/或 F-停泊位点。各种支架蛋白，包括 KSR1/2、IQGAP1、MP1和β-Arrestin1/2，也参与ERK1/2 MAP激酶级联的调节。Ras-Raf-MEK-ERK 信号传导活性在大约三分之一的人类癌症中被上调，而针对该信号传导途径的成分的靶向抑制代表了一种流行的抗癌策略。
观察值〜44/42 kDa。计算值为41.26/36.30 kDa（人MAPK1亚型1/2），41.14 kDa（大鼠MAPK1），41.14 kDa（小鼠MAPK1），43.00/38.14 kDa（人MAPK3亚型1/2），42.95/45.64 kDa（大鼠MAPK3亚型1/2），42.93 kDa（小鼠MAPK3）（已删除Met1）。 靶序列在人MAPK3剪接亚型 3（ERK1b）中不存在。
Immunogen
与大鼠MAPK 3（ ERK-1）的C末端序列相对应的KLH偶联的线性肽。
表位：C端
Other Notes
替代品：06-182
浓度：请参考批次特异性浓缩物的分析证书。
Physical form
在含 0.1 M Tris-甘氨酸（pH 7.4），150 mM NaCl 和 0.05％ 叠氮化钠的缓冲液中的纯化的兔多克隆抗体。
形式：纯化
纯化的蛋白A。
Preparation Note
自收到之日起，在 2-8°C 条件下可稳定保存1年
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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