ABT146
抗闭合蛋白抗体
from rabbit, purified by affinity chromatography
别名:
Occludin
登录 查看组织和合同定价。
选择尺寸
关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
WB
Citations:
8
conjugate
unconjugated
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
species reactivity (predicted by homology)
bovine (based on 100% sequence homology), mouse (based on 100% sequence homology), porcine (based on 100% sequence homology), canine (based on 100% sequence homology), primate (based on 100% sequence homology), rat (based on 100% sequence homology), equine (based on 100% sequence homology)
packaging
antibody small pack of 25 μg
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Quality Level
Gene Information
human ... OCLN(4950), OCLN(100506658)
General description
观测值〜63 kDa。计算出的分子量为 63 kDa，但是封闭蛋白在蛋白质印迹中的观察到的分子量范围为 ~55 至 ~65 kDa（Florian, P., et al. (2003).Biochemical and Biophysical Research Communications.304:5-10）。
闭锁蛋白是位于紧密连接的 65 kDa 完整的血浆膜蛋白。它是细胞因子诱导的紧密连接的细胞旁渗透屏障调节所必需的。人们相信闭锁蛋白阻止大分子通过紧密连接的运输过程。闭锁蛋白由 OLCN 基因编码，横跨质膜四次，形成两个胞外环，并将其 NH2 和 COOH 末端暴露于胞质中。该胞质尾巴直接结合至 ZO 家族蛋白，该家族将蛋白复合物连接至肌动蛋白细胞骨架。磷酸化/去磷酸化在调节闭锁蛋白和紧密连接中起主要作用。闭锁蛋白的表达是丙型肝炎病毒（HCV）感染肝细胞所必需的。
Immunogen
与人封闭蛋白 N 末端相对应的 KLH 偶联的线性肽。
表位：N端
Application
研究子类别
ECM 蛋白
ECM 蛋白
研究类别
细胞结构
细胞结构
该抗封闭蛋白抗体经验证可用于蛋白质印迹法检测封闭蛋白。
Biochem/physiol Actions
该抗体识别闭锁蛋白的 N 端。
Physical form
亲和纯化
在含 0.1 M Tris-甘氨酸（pH 7.4），150 mM NaCl 和 0.05％ 叠氮化钠的缓冲液中的纯化的兔多克隆抗体。
Preparation Note
自收到之日起，在 2-8°C 条下可稳定保存 1 年。
Analysis Note
对照
人肾组织裂解液
人肾组织裂解液
通过蛋白质印迹在人肾组织裂解液中进行了评估。
蛋白质印迹分析：0.2 µg/mL 的该抗体在 10 µg 的人肾脏组织裂解液中检测到了封闭蛋白。
蛋白质印迹分析：0.2 µg/mL 的该抗体在 10 µg 的人肾脏组织裂解液中检测到了封闭蛋白。
Other Notes
浓度：请参考批次特异性浓缩物的分析证书。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
未找到合适的产品？
试试我们的产品选型工具.
Hengameh Chloé Mirsepasi-Lauridsen et al.
Clinical and translational gastroenterology, 7, e149-e149 (2016-03-05)
The potential of Escherichia coli (E. coli) isolated from inflammatory bowel disease (IBD) patients to damage the integrity of the intestinal epithelium was investigated. E. coli strains isolated from patients with ulcerative colitis (UC) and healthy controls were tested for
Duobin Zhang et al.
Journal of leukocyte biology, 111(5), 953-966 (2021-09-30)
Ischemic stroke (IS) can cause disability and death, and microglia as the immune component of the CNS can release inflammatory factors and participate in blood-brain barrier (BBB) dysfunction. This study aimed to investigate the effects of long noncoding RNA (lncRNA)
Amy L Creekmore et al.
Pain, 159(9), 1777-1789 (2018-06-19)
In humans, chronic psychological stress is associated with increased intestinal paracellular permeability and visceral hyperalgesia, which is recapitulated in the chronic intermittent water avoidance stress (WAS) rat model. However, it is unknown whether enhanced visceral pain and permeability are intrinsically
Diana Duarte Lobo et al.
Acta neuropathologica communications, 8(1), 152-152 (2020-09-02)
Blood-brain barrier (BBB) disruption is a common feature in neurodegenerative diseases. However, BBB integrity has not been assessed in spinocerebellar ataxias (SCAs) such as Machado-Joseph disease/SCA type 3 (MJD/SCA3), a genetic disorder, triggered by polyglutamine-expanded ataxin-3. To investigate that, BBB
Maj Schneider Thomsen et al.
PloS one, 16(3), e0236770-e0236770 (2021-03-13)
The blood-brain barrier (BBB) is formed by brain capillary endothelial cells (BECs) supported by pericytes and astrocytes. The BBB maintains homeostasis and protects the brain against toxic substances circulating in the blood, meaning that only a few drugs can pass
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持