biological source
mouse
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
10H, monoclonal
assay
≥95% (SDS-PAGE)
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human, Drosophila, rat, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(Immunoblotting (2.5 µg/mL)
Immunocytochemistry (5-20 µg/mL))
isotype
IgG3
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
蛋白 A 纯化的小鼠单克隆抗体。识别聚ADP-核糖。
识别依托泊苷处理的HL-60细胞中的聚(ADP)-核糖(PAR)。
该抗PAR(Ab-1)小鼠mAb(10H)经验证可用于免疫印迹、免疫细胞化学检测PAR(Ab-1)。
Immunogen
牛
纯化的聚(ADP-核糖)
Application
免疫印迹(2.5 µg/ml)
免疫细胞化学(5-20 µg/ml)
免疫细胞化学(5-20 µg/ml)
Packaging
请参考特定浓度批号的标签。
Physical form
在100 mM NaCl,50 mM HEPES,1% BSA,pH 7.4中。
Preparation Note
初次融化后,等分并冷冻(-20°C)。
Analysis Note
阳性对照
依托泊苷处理的HL-60细胞
依托泊苷处理的HL-60细胞
阴性对照
正常小鼠IgG
正常小鼠IgG
Other Notes
Kawamitsu, H., et al. 1984.Biochemistry23, 3771.
用67 µM依托泊苷处理3.5 h可诱导HL-60细胞中的PAR。应滴定抗体,以在单个系统中获得最佳结果。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性:标准处理(A)
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable