AP1064
PhosphoDetect 抗-PDH-E1α (pSer³⁰⁰)兔多克隆抗体
liquid, Calbiochem®
别名:
抗丙酮酸脱氢酶pSer³⁰⁰兔pAb
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
human
Application:
Citations:
32
产品名称
PhosphoDetect 抗-PDH-E1α (pSer³⁰⁰)兔多克隆抗体, liquid, Calbiochem®
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pSer300)
Quality Level
Analysis Note
对照
HEK293细胞
HEK293细胞
阴性对照
用二氯乙酸盐处理的HEK293细胞
用二氯乙酸盐处理的HEK293细胞
Application
免疫印迹（0.5μg/ml）
免疫细胞化学（1μg/ml）
Disclaimer
毒性：标准处理（A）
General description
免疫亲和纯化的兔多克隆抗体。可识别出在Ser300处磷酸化的~44 kDa的PDH-E1α蛋白。
识别HEK293细胞中在Ser300磷酸化的~44 kDa PDH-E1α蛋白。
该PhosphoDetect抗-PDH-E1α pSer³⁰⁰）兔pAb经验证可用于免疫印迹法、免疫细胞化学检测PDH-E1α（pSer³⁰⁰）。
Immunogen
人
合成磷酸肽，对应于人PDH-E1的Ser³⁰⁰磷酸化位点周围的氨基酸α
Other Notes
Rardin M.J., et. al. 2009.Anal.Biochem.2, 157.
Seifert, F., et al. 2007.Biochemistry 21, 6277.
Lee, J., et al. 2007.Mol.Cell Prot.4, 669.
Patel, M.S. and Korotchkina, L.G.2006 Biochem.Soc.Trans.34, 217.
Korotchkina, L.G., et al. 2001.J. Biol. Chem. 40, 37223.
Seifert, F., et al. 2007.Biochemistry 21, 6277.
Lee, J., et al. 2007.Mol.Cell Prot.4, 669.
Patel, M.S. and Korotchkina, L.G.2006 Biochem.Soc.Trans.34, 217.
Korotchkina, L.G., et al. 2001.J. Biol. Chem. 40, 37223.
由于高程度序列一致性，该抗体也预期与小鼠，大鼠，牛，斑马鱼和非洲爪蟾发生交叉反应。磷酸化PDH-E1α抗体应进行滴定以在单个系统中获得最佳结果。
Packaging
请参考特定浓度批号的标签。
Physical form
在150 mM NaCl，100 mM Tris-甘氨酸，pH 7.4中。
Preparation Note
初次融化后，等分并冷冻（-20°C）。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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