产品名称
山羊抗人IgG抗体,HRP偶联, Chemicon®, from goat
biological source
goat
conjugate
peroxidase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
lyophilized
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
western blot: suitable
isotype
IgG
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Physical form
使用与琼脂糖珠偶联的抗原通过免疫亲和层析纯化。冻干。缓冲液为0.01 M磷酸钠,0.25 M NaCl,pH值7.6和15 mg/ml BSA。不含防腐剂。
General description
免疫球蛋白G(IgG)属于免疫球蛋白家族,是一种广泛表达的血清抗体。这是一种糖蛋白,主要帮助免疫防御系统。大约70%的总免疫球蛋白由IgG组成。IgG是一种单体免疫球蛋白,由四条多肽链组成。它由两条50 kDa重链(γ)和两条25 kDa轻链组成。这些链通过二硫键相互连接。每个分子有两个抗原结合位点。IgG是最丰富的免疫球蛋白。它在血液和组织液中的分布大致相等,占人血清免疫球蛋白的75%。有4个子类:IgG1(66%),IgG2(23%),IgG3(7%)和IgG4(4%)。
Immunogen
由纯化人IgG制备。
Other Notes
研究子类别
完整免疫球蛋白二抗
完整免疫球蛋白二抗
研究类别:二级&对照抗体
Application
山羊抗人IgG抗体,HRP偶联物已用于:
- 在酶联免疫吸附测定(ELISA)中检测结合的免疫球蛋白G(IgG)
- 在免疫组化或细胞化学中,稀释度为1:500-1:5,000
- 用于稀释度为1:5,000-1:100,000的显色底物和稀释度为的1:10,000-1:200,000的ECL底物的蛋白质印迹。
- 在EIA中,稀释度为1:5,000-1:100,000
Biochem/physiol Actions
免疫球蛋白G(IgG)参与II型和III型超敏反应。它启动补体系统的经典途径,也中和病毒颗粒和毒素。母体IgG可通过人胎盘传递给胎儿,为新生儿抵抗感染提供免疫防御。它促进抗体依赖性细胞介导的细胞毒性(ADCC)。IgG被木瓜蛋白酶消化产生抗原结合片段(Fab)。IgG的胃蛋白酶消化导致可结晶片段(Fc)的形成。严重急性呼吸综合征冠状病毒(SARS-CoV)-2感染患者的IgG水平可能有助于评估2019冠状病毒病(COVID-19)的严重程度和预后。
该抗体与人IgG的重链和大多数人免疫球蛋白共有的轻链反应。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
Preparation Note
在接收之日起,将冻干产品在产物2°-8°C下保存长达12个月。复溶后,产品作为未稀释的液体在2°-8°C下稳定6周。用2 mL蒸馏水复溶小瓶。要延长复溶后溶液的保存时间,请添加等体积的甘油,使甘油的最终浓度达到 50%,然后以未稀释的等分试样置于 -20°C 下可保存长达 12 个月。请注意,添加甘油后,抗体(和缓冲盐)的浓度将降低至原浓度的一半,并且必须相应地调整最终的工作稀释度。应避免反复冻融循环。
警告:
使用叠氮化钠作为防腐剂将显著抑制HRP的酶活性。
警告:
使用叠氮化钠作为防腐剂将显著抑制HRP的酶活性。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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