biological source
goat
conjugate
peroxidase conjugate
antibody form
affinity purified immunoglobulin
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
rabbit
manufacturer/tradename
Chemicon®
concentration
1 mg/mL (after reconstitution)
technique(s)
ELISA: suitable, immunohistochemistry: suitable, western blot: suitable
isotype
IgG
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
General description
亲和纯化的山羊抗兔,HRP偶联物。
Application
ELISA和蛋白质印迹:
可用1:5,000-1:100,000稀释度。
免疫组化:
可用1:500-1:5,000稀释度。
最佳工作稀释度必须由最终用户确定。
可用1:5,000-1:100,000稀释度。
免疫组化:
可用1:500-1:5,000稀释度。
最佳工作稀释度必须由最终用户确定。
研究子范畴
完整免疫球蛋白二抗
完整免疫球蛋白二抗
研究范畴
二抗&对照抗体
二抗&对照抗体
该山羊抗兔IgG抗体,过氧化物酶偶联物经验证可用于ELISA、IH、WB法检测兔IgG。
Biochem/physiol Actions
对兔IgG重链和轻链具有特异性。
Physical form
免疫亲和纯化
在含有0.01M PBS,pH 7.1和15mg/mL BSA的缓冲液中与辣根过氧化物酶偶联的纯化山羊IgG。冻干。
复溶:
用无菌蒸馏水复溶以达到小瓶标签上指示的体积。
复溶:
用无菌蒸馏水复溶以达到小瓶标签上指示的体积。
Preparation Note
自装运之日起,冻干产品在2-8°C可最多保存12个月。
复溶后,产品作为未稀释的液体在2-8°C下稳定数周。要延长重悬后溶液的保存时间,请添加等体积的甘油,使甘油的终浓度达到 50%,然后以未稀释的等分试样置于 -20°C 下可保存长达 12 个月。请注意,添加甘油后,蛋白质(和缓冲盐)的浓度将降至原浓度的一半。应避免反复冻/融循环。
警告:使用叠氮化钠作为防腐剂将显著抑制HRP的酶活性。
复溶后,产品作为未稀释的液体在2-8°C下稳定数周。要延长重悬后溶液的保存时间,请添加等体积的甘油,使甘油的终浓度达到 50%,然后以未稀释的等分试样置于 -20°C 下可保存长达 12 个月。请注意,添加甘油后,蛋白质(和缓冲盐)的浓度将降至原浓度的一半。应避免反复冻/融循环。
警告:使用叠氮化钠作为防腐剂将显著抑制HRP的酶活性。
Other Notes
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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