biological source
donkey
conjugate
peroxidase conjugate
antibody form
purified immunoglobulin
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
goat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunohistochemistry: suitable, western blot: suitable
isotype
IgG
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
General description
免疫球蛋白 G(IgG)是人血清中最丰富的蛋白质之一,成人血液中的正常水平在 8-17 mg/mL 之间。IgG对我们抵抗微生物很重要,而B淋巴细胞产生的分子是我们适应性免疫应答的一部分。IgG 分子具有两个独立的功能;与引起反应的病原体结合,以及募集其他细胞和分子来破坏抗原。IgG 池的变异性是通过体细胞重组产生的,在给定时间点个体的特异性数量估计为 1011 个变异体。
Application
免疫组化:
可用1:500-1:5,000稀释度。
最佳工作稀释度必须由最终用户确定。
可用1:500-1:5,000稀释度。
最佳工作稀释度必须由最终用户确定。
驴抗山羊IgG抗体,HRP偶联物,吸附物种是一种用于ELISA、IH &WB的抗驴山羊IgG的抗体。
Physical form
在冻干缓冲液0.01 M磷酸钠,0.25 M NaCl,pH值7.6和15 mg/mL BSA中的纯化的驴二抗IgG。
复溶:
用500 μL无菌蒸馏水复溶。
复溶:
用500 μL无菌蒸馏水复溶。
Preparation Note
将冻干产品在2-8°C下保存长达12个月。
复溶后,产品作为未稀释的液体在2-8°C下稳定数周。要延长复溶后溶液的保存时间,请添加等体积的甘油,使甘油的最终浓度达到 50%,然后以未稀释的等分试样置于 -20°C 下可保存长达 12 个月。请注意,添加甘油后,蛋白质(和缓冲盐)的浓度将降至原浓度的一半。应避免反复冻/融循环。
警告:使用叠氮化钠作为防腐剂将显著抑制HRP的酶活性。
复溶后,产品作为未稀释的液体在2-8°C下稳定数周。要延长复溶后溶液的保存时间,请添加等体积的甘油,使甘油的最终浓度达到 50%,然后以未稀释的等分试样置于 -20°C 下可保存长达 12 个月。请注意,添加甘油后,蛋白质(和缓冲盐)的浓度将降至原浓度的一半。应避免反复冻/融循环。
警告:使用叠氮化钠作为防腐剂将显著抑制HRP的酶活性。
Analysis Note
酶联免疫吸附试验已常规检验。
ELISA和蛋白质印迹:
可用1:5,000-1:100,000稀释度。
ELISA和蛋白质印迹:
可用1:5,000-1:100,000稀释度。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
11 - Combustible Solids
wgk
WGK 3
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