biological source
goat
Quality Level
conjugate
peroxidase conjugate
antibody form
F(ab′)2 fragment of affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
mouse
manufacturer/tradename
Chemicon®
concentration
0.8 mg/mL
technique(s)
ELISA: suitable, western blot: suitable
shipped in
wet ice
target post-translational modification
unmodified
General description
免疫球蛋白 G（IgG）是人血清中最丰富的蛋白质之一，成人血液中的正常水平在 8-17 mg/mL 之间。IgG 对我们抵抗微生物很重要，而 B 淋巴细胞产生的分子是我们适应性免疫反应的一部分。IgG 分子具有两个独立的功能；与引起反应的病原体结合，以及募集其他细胞和分子来破坏抗原。IgG 池的变异性是通过体细胞重组产生的，在给定时间点个体的特异性数量估计为 1011 个变异体。
Application
EIA和蛋白质印迹：1：5,000-1：100,000。
免疫组化：1：500-1：5,000。
最佳工作稀释度必须由最终用户进行确定。
免疫组化：1：500-1：5,000。
最佳工作稀释度必须由最终用户进行确定。
研究子类别
用于双重标记的吸附的二抗
用于双重标记的吸附的二抗
研究类别
二抗&对照抗体
二抗&对照抗体
该山羊抗小鼠IgG抗体，HRP偶联物，吸附物种经验证可用于ELISA，WB检测小鼠IgG。
Physical form
冻干。缓冲液=0.01 M磷酸钠，0.25M NaCl，pH 7.6和15 mg/mL BSA。
复溶：
用500 μL无菌蒸馏水复溶。
复溶：
用500 μL无菌蒸馏水复溶。
Preparation Note
将冻干产品在2-8°C下保存长达12个月。复溶后，产品作为未稀释的液体在2-8°C下稳定数周。要延长复溶后溶液的保存时间，请添加等体积的甘油，使甘油的最终浓度达到 50％，然后以未稀释的等分试样置于 -20°C 下可保存长达 12 个月。请注意，添加甘油后，蛋白质（和缓冲盐）的浓度将降至原浓度的一半。应避免反复冻/融循环。
警告：
使用叠氮化钠作为防腐剂将显著抑制HRP的酶活性。
警告：
使用叠氮化钠作为防腐剂将显著抑制HRP的酶活性。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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|---|---|
| AP181P | 04053252675713 |
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