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Merck
CN

AP182B

驴抗兔IgG抗体,生物素-SP偶联物,物种吸附

Chemicon®, from donkey

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UNSPSC Code:
12352203
NACRES:
NA.46
eCl@ss:
32160702
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产品名称

驴抗兔IgG抗体,生物素-SP偶联物,物种吸附, Chemicon®, from donkey

biological source

donkey

conjugate

biotin conjugate

antibody form

affinity purified immunoglobulin

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

rabbit

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

Application

EIA:1:50,000-1:100,000使用酶结合链霉亲和素的蛋白质印迹:1:10,000-1:100,000。免疫组化:1:500-1:5,000 流式细胞术:1:200-1:1,000荧光免疫组化/细胞化学:1:200-1:1,1000 最佳工作稀释度必须由最终用户确定。
研究子类别
用于双重标记的吸附的二抗
研究范畴
二抗&对照抗体
驴抗兔IgG抗体,生物素-SP偶联物,吸附物种可检测驴兔IgG&水平已发表&,经验证可用于ELISA &WB。

Biochem/physiol Actions

兔IgG

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

免疫球蛋白 G(IgG)是人血清中最丰富的蛋白质之一,成人血液中的正常水平在 8-17 mg/mL 之间。IgG 对我们抵抗微生物很重要,而 B 淋巴细胞产生的分子是我们适应性免疫应答的一部分。IgG 分子具有两个独立的功能;与引起反应的病原体结合,以及募集其他细胞和分子来破坏抗原。IgG 池的变异性是通过体细胞重组产生的,在给定时间点个体的特异性数量估计为 1011 个变异体。

Physical form

冻干。缓冲液=0.01 M磷酸钠,0.15 M NaCl,pH值7.1,含15 mg/ml BSA和0.1%叠氮化钠。

复溶:

用500 μL无菌蒸馏水复溶。

Preparation Note

将冻干产品在2-8°C下保存长达12个月。复溶后,产品作为未稀释的液体在2-8°C下稳定数周。要延长复溶后溶液的保存时间,请添加等体积的甘油,使甘油的最终浓度达到 50%,然后以未稀释的等分试样置于 -20°C 下可保存长达 12 个月。请注意,添加甘油后,蛋白质(和缓冲盐)的浓度将降至原浓度的一半。应避免反复冻/融循环。

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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hcodes

Hazard Classifications

Aquatic Chronic 3

存储类别

11 - Combustible Solids

wgk

WGK 3


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商品

荧光Western blot检测因具有多重检测的方便性而变得越来越受研究者的欢迎,但这种检测仍需要专门优化的工具来获得理想的实验结果。这里我们推荐一个最佳组合,以帮助大家在荧光检测中快速获得稳定、易重复的数据。

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Western blotting is one of the most commonly used techniques in the lab, yet difficulties persist in obtaining consistent, quality results. We’ve been helping scientists publish their Western blots for decades, with continued innovation and steadfast technical support. Explore our expanded portfolio of products, including optimized reagents for chemiluminescent and Ḁuorescent Westerns, as well as the SNAP i.d.® system, which reduces blocking, washing and antibody incubation time from hours to minutes.

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