产品名称
驴抗兔IgG抗体,HRP偶联物,吸附物种, Chemicon®, from donkey
biological source
donkey
conjugate
peroxidase conjugate
antibody form
affinity purified immunoglobulin
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
rabbit
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
western blot: suitable
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Application
EIA和蛋白质印迹:1:5,000-1:100,000。
免疫组化:1:500-1:5,000。
以ECL底物进行的蛋白质印迹:1:10,000-1:200,000。
最佳工作稀释度必须由最终用户进行确定。
免疫组化:1:500-1:5,000。
以ECL底物进行的蛋白质印迹:1:10,000-1:200,000。
最佳工作稀释度必须由最终用户进行确定。
研究子类别
用于双重标记的吸附的二抗
用于双重标记的吸附的二抗
研究范畴
二抗&对照抗体
二抗&对照抗体
驴抗兔IgG抗体(HRP偶联物,吸附物种)是一种针对驴兔IgG的抗体,用于ELISA &WB。
Biochem/physiol Actions
兔IgG
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
免疫球蛋白 G(IgG)是人血清中最丰富的蛋白质之一,成人血液中的正常水平在8-17 mg/mL之间。IgG对我们抵抗微生物很重要,而B淋巴细胞产生的分子是我们适应性免疫反应的一部分。IgG分子具有两个独立的功能;与引起反应的病原体结合,以及募集其他细胞和分子来破坏抗原。IgG池的变异性是通过体细胞重组产生的,在给定时间点个体的特异性数量估计为1011个变异体。
Other Notes
浓度:请参考批次特异性浓缩物的检验报告。
Physical form
冻干。缓冲液=0.01 M磷酸钠,0.25 M NaCl,pH值7.6和15 mg/ml BSA。不含防腐剂。
复溶:
用500 μL无菌蒸馏水复溶。
复溶:
用500 μL无菌蒸馏水复溶。
Preparation Note
将冻干产品在2-8°C下保存长达12个月。复溶后,产品作为未稀释的液体在2-8°C下稳定数周。要延长复溶后溶液的保存时间,请添加等体积的甘油,使甘油的最终浓度达到 50%,然后以未稀释的等分试样置于 -20°C 下可保存长达 12 个月。请注意,添加甘油后,蛋白质(和缓冲盐)的浓度将降至原浓度的一半。应避免反复冻/融循环。
警告:
使用叠氮化钠作为防腐剂将显著抑制HRP的酶活性。
警告:
使用叠氮化钠作为防腐剂将显著抑制HRP的酶活性。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
11 - Combustible Solids
wgk
WGK 3
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