登录 查看组织和合同定价。
选择尺寸
关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
BMK13, monoclonal
Application:
ELISA
immunohistochemistry
western blot
immunohistochemistry
western blot
Species reactivity:
human
Citations:
5
Technique(s):
ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
Uniprot accession no.:
产品名称
嗜酸性粒细胞主要碱性蛋白抗体,克隆BMK13, clone BMK13, Chemicon®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
BMK13, monoclonal
species reactivity
human
should not react with
horse, mouse, rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PRG2(5553)
Application
免疫组化:克隆BMK-13可与新鲜冷冻组织反应,将组织切片(6μm),在室温下干燥1小时,用甲醇和丙酮1:1的溶液固定,然后风干1小时。 使用前,可将切片用铝箔包裹,-70°C储存。 对于石蜡包埋切片,进行皮肤活检应使用4%PFA(3小时)。 将5 μM的切片脱蜡,然后用水水化。 在封闭和抗体染色前,应先用0.1%胰蛋白酶水溶液处理15分钟。该抗体在冷冻组织中的反应比在石蜡包埋组织中更好。
蛋白质印迹:将纯化的正常人嗜酸性粒细胞用洋地黄皂苷(5mg/mL)处理10分钟,然后在样品缓冲液中煮沸,并在15%SDS-PAGE上进行电泳。 纯化的MBP迁移至14kDa,典型的人类样本的范围为10-16kDa。
ELISA开发用于估算MBP。
最佳工作稀释度必须由最终用户进行确定。
蛋白质印迹:将纯化的正常人嗜酸性粒细胞用洋地黄皂苷(5mg/mL)处理10分钟,然后在样品缓冲液中煮沸,并在15%SDS-PAGE上进行电泳。 纯化的MBP迁移至14kDa,典型的人类样本的范围为10-16kDa。
ELISA开发用于估算MBP。
最佳工作稀释度必须由最终用户进行确定。
嗜酸性粒细胞主要碱性蛋白抗体(克隆BMK13)是一种针对嗜酸性粒细胞主要碱性蛋白的抗体,可用于ELISA、WB、IH(P)。
研究子类别
免疫球蛋白 & 免疫学
免疫球蛋白 & 免疫学
研究类别
炎症 & 免疫学
炎症 & 免疫学
Biochem/physiol Actions
CBL419对嗜酸性粒细胞主要碱性蛋白具有特异性,与嗜酸性粒细胞活化阶段无关,可视为“泛嗜酸性粒细胞”标志物。该抗体可识别在孕妇胎盘和血清中发现的23.8 kDa的MBP前体(妊娠相关MBP),以及在嗜酸性粒细胞大特异性颗粒基质中发现的13.8 kDa的成熟形式。它不与马、小鼠或大鼠的MBP交叉反应。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
Other Notes
浓度:请参考批次特异性浓缩物的检验报告。
Physical form
形式:纯化
该单克隆抗体溶于含有10mM叠氮化钠和1mg/ml牛血清白蛋白的磷酸盐缓冲液中,浓度为 100μg/ml。 我们建议每个实验室确定在其特定应用中使用的最佳工作滴定度。
Preparation Note
若购买后1个月内使用,则储存于+4°C,若要长期储存,则将抗体等分成小体积并储存于-20°C。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
未找到合适的产品?
试试我们的产品选型工具.
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Toru Sonoyama et al.
International journal of molecular sciences, 23(21) (2022-11-12)
Transglutaminase (TGM) isoform catalyze the cross-linking reaction of identical or different substrate proteins. Eosinophil has been recognized in chronic rhinosinusitis with nasal polyps (CRSwNP) forming tissue eosinophil in nasal polyp (NP), and TGM isoforms are suggested to be associated with
Takahiro Kawakami et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 34(6), 960-969 (2018-10-17)
This study aimed to investigate the contribution of a proliferation-inducing ligand (APRIL), a member of the tumor necrosis factor (TNF) superfamily implicated in plasma cell survival, to the development of plasma cell-rich lesions in immunoglobulin G4-related disease (IgG4-RD). We performed
ACE2 downregulation in olfactory mucosa: Eosinophilic rhinosinusitis as COVID-19 protective factor?
Concepció Marin et al.
Allergy, 76(9), 2904-2907 (2021-05-15)
Hironobu Nishijima et al.
Laboratory investigation; a journal of technical methods and pathology, 96(9), 981-993 (2016-06-21)
The posterior nasal nerve is the dominant source of the parasympathetic, sympathetic, and sensory fibers that innervate the nasal respiratory mucosa. Therefore, a posterior nasal neurectomy (PNN) is thought to induce denervation of the nasal mucosa and relieve the nasal
Alba Garrido-Trigo et al.
Nature communications, 14(1), 4506-4506 (2023-07-27)
Ulcerative colitis and Crohn's disease are chronic inflammatory intestinal diseases with perplexing heterogeneity in disease manifestation and response to treatment. While the molecular basis for this heterogeneity remains uncharacterized, single-cell technologies allow us to explore the transcriptional states within tissues
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持