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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
10F12.2, monoclonal
Application:
ELISA
immunocytochemistry
immunohistochemistry
western blot
immunocytochemistry
immunohistochemistry
western blot
Species reactivity:
human
Citations:
39
Technique(s):
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
Uniprot accession no.:
产品名称
抗色素上皮衍生的因子抗体,克隆10F12.2, clone 10F12.2, Chemicon®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
10F12.2, monoclonal
species reactivity
human
species reactivity (predicted by homology)
rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... SERPINF1(5176)
Analysis Note
对照
眼组织
眼组织
已通过蛋白质印迹对人体胎盘素裂解物进行了常规评估。
蛋白质印迹分析:
该批次的1:1000的稀释液在 10μg 人体胎盘素裂解物中检测到色素上皮。
蛋白质印迹分析:
该批次的1:1000的稀释液在 10μg 人体胎盘素裂解物中检测到色素上皮。
Application
抗色素上皮衍生因子抗体,克隆10F12.2是一种抗色素上皮衍生因子的抗体,用于ELISA、IC、IH &WB。
研究子类别
神经化学&神经营养因子
神经化学&神经营养因子
研究类别
神经科学
神经科学
蛋白质印迹:
当针对免疫原进行测试时,可识别分子量约为46 kDa的蛋白。可获得蛋白质印迹阳性对照(目录号AG672)
ELISA:
前一批抗体用于ELISA。
免疫细胞化学:
前一批抗体用于免疫细胞化学。
免疫组化:
前一批抗体用于冰冻切片。
最佳的工作稀释度必须由最终用户确定。
当针对免疫原进行测试时,可识别分子量约为46 kDa的蛋白。可获得蛋白质印迹阳性对照(目录号AG672)
ELISA:
前一批抗体用于ELISA。
免疫细胞化学:
前一批抗体用于免疫细胞化学。
免疫组化:
前一批抗体用于冰冻切片。
最佳的工作稀释度必须由最终用户确定。
Biochem/physiol Actions
识别色素上皮衍生因子(PEDF)。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
~46 kDa
色素上皮衍生因子(PEDF)首先被鉴定为在培养的胎儿视网膜色素上皮(RPE)细胞的条件培养基中分泌的50 kDa蛋白(Tombran-Tink,1991)。最近,发现PEDF在体内视网膜中具有神经营养活性(Cayouette,1999)。PEDF也是其他系统中的神经营养和神经保护因子,包括培养的小脑颗粒细胞、原代海马神经元、脊髓运动神经元和在没有RPE细胞的情况下培养的两栖动物感光细胞(Karakousis,2001)。PEDF与视网膜母细胞瘤和小脑颗粒细胞的结合可能是受体介导的。最近,PEDF在内皮细胞培养物中以及在大鼠角膜和体内视网膜中被证明是一种有效的血管生成抑制剂(Dawson,1999;Stellmach,2001)。
Immunogen
重组人GST-PEDF融合蛋白。在大肠埃希菌中表达的序列对应于在N-末端用GST标记的人PEDF的氨基
酸196-347。SDS-PAGE上的GST-PEDF约为46 kDa。
酸196-347。SDS-PAGE上的GST-PEDF约为46 kDa。
Other Notes
浓度:请参考批次特异性浓缩物的分析证书。
Physical form
形式:纯化
纯化的小鼠单克隆IgG2a,溶于含有0.02 M PBS,pH 7.6,0.25 mM NaCl,和0.1%叠氮化钠的缓冲液中。
纯化的蛋白 A
Preparation Note
自收到之日起以未稀释的等分试样形式,在2-8°C可稳定保存6个月。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Daisy S Nakamura et al.
BMC musculoskeletal disorders, 18(1), 39-39 (2017-01-27)
Inflammation is a major cause of cartilage destruction and leads to the imbalance of metabolic activities in the arthritic joint. Pigment epithelium-derived factor (PEDF) has been reported to have both pro- and anti-inflammatory activities in various cell types and to
Terakeith Lertsburapa et al.
Journal of neuroscience research, 75(5), 624-631 (2004-03-03)
Pigment epithelium-derived factor (PEDF) is a multifunctional protein with known anti-angiogenic and trophic properties, capable of promoting the survival and growth of Schwann cells (SC). Normal rat SCs and ganglioneuroma-derived human SCs secrete PEDF. The ability of normal SC to
Célia Parinot et al.
International journal of molecular sciences, 25(12) (2024-06-27)
Among the myriad of existing tyrosine kinase receptors, the TAM family-abbreviated from Tyro3, Axl, and Mer tyrosine kinase (MerTK)-has been extensively studied with an outstanding contribution from the team of Prof. Greg Lemke. MerTK activity is implicated in a wide
Pigment Epithelium-Derived Factor Plays a Role in Alzheimer's Disease by Negatively Regulating Aβ42.
Mao Huang et al.
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics, 15(3), 728-741 (2018-05-08)
Alzheimer's disease (AD) is the most common cause of dementia. Pigment epithelium-derived factor (PEDF), a unique neurotrophic protein, decreases with aging. Previous reports have conflicted regarding whether the PEDF concentration is altered in AD patients. In addition, the effect of
Shou-Chuan Shih et al.
PloS one, 11(7), e0157647-e0157647 (2016-07-08)
Fibrogenesis is induced by repeated injury to the liver and reactive regeneration and leads eventually to liver cirrhosis. Pigment epithelium derived factor (PEDF) has been shown to prevent liver fibrosis induced by carbon tetrachloride (CCl4). A 44 amino acid domain
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