产品名称
抗-HLA-B27,Cln HLA-ABC-m3（小鼠单克隆）, clone HLA-ABC-m3, Chemicon®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
HLA-ABC-m3, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... HLA-B(3106)
Application
该抗HLA-B27抗体（克隆HLA-ABC-m3）经验证可用于FC、IC检测HLA-B27。
该抗体可用于强直性脊柱炎和前葡萄膜炎等疾病中HLA-B27的检查。
建议使用 稀释度
1.流式细胞术和间接免疫荧光1：60~1：120
用等渗缓冲液稀释。在100μl缓冲液中，每1 x 10E6外周血单核细胞（PBMC）使用50 μl稀释的抗体。
2.细胞毒性试验1：25。
建议使用 稀释度
1.流式细胞术和间接免疫荧光1：60~1：120
用等渗缓冲液稀释。在100μl缓冲液中，每1 x 10E6外周血单核细胞（PBMC）使用50 μl稀释的抗体。
2.细胞毒性试验1：25。
Biochem/physiol Actions
该抗体与HLA-B27抗原牢固结合。它与HLA-B7抗原的结合强度也较低。通过斯卡查德分析进行的亲和力研究表明，该抗体对HLA-B27（9.7 x108M-1）的亲和力高于对HLA-B7
（9.5 x107M-1）的亲和力。
细胞反应性：
HLA-B27淋巴细胞染色的流式细胞术分析得出以下结果：
受试者 荧光强度
杂合HLA-B27+ 强
纯合HLA-B7+ 强
杂合HLA-B7+ 微弱的
非B27，非B7 阴性
（9.5 x107M-1）的亲和力。
细胞反应性：
HLA-B27淋巴细胞染色的流式细胞术分析得出以下结果：
受试者 荧光强度
杂合HLA-B27+ 强
纯合HLA-B7+ 强
杂合HLA-B7+ 微弱的
非B27，非B7 阴性
General description
之前分配的蛋白标识符P03989已合并到P01889中。完整的详细资料见于UniProt数据库。
Other Notes
浓度：请参考批次特异性浓缩物的分析证书。
Physical form
形式：纯化
抗体以含有0.2%牛血清白蛋白和0.1%叠氮化钠的磷酸盐缓冲盐水（pH 7.4）提供。 通过流式细胞仪检测每个批次的特性。
Preparation Note
保存于2-8°C。对于延长的周期，以未稀释的等分试样储存在-20°C以下。应避免反复冻/融循环。
警告：单克隆试剂溶液含有0.1%叠氮化钠作为防腐剂。由于该物料在管道中积聚会产生潜在危害，因此应将废试剂与大量水一起处置。
警告：单克隆试剂溶液含有0.1%叠氮化钠作为防腐剂。由于该物料在管道中积聚会产生潜在危害，因此应将废试剂与大量水一起处置。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Christoffer Dellgren et al.
Journal of immunology (Baltimore, Md. : 1950), 197(12), 4807-4816 (2016-11-09)
HLA class I cell surface expression is crucial for normal immune responses, and variability in HLA expression may influence the course of infections. We have previously shown that classical HLA class I expression on many human cell types is biased
Barbara Neerinckx et al.
RMD open, 3(1), e000451-e000451 (2017-09-08)
The strong genetic association between HLA-B27 and ankylosing spondylitis has been known for over 40 years. HLA-B27 positivity is possibly associated with severity of ankylosis. We studied the in vitro and in vivo impact of HLA-B27 in models of chondrogenesis
Shengli Song et al.
Frontiers in immunology, 12, 705140-705140 (2021-07-31)
Antibody-mediated allograft rejection (AMR) causes more kidney transplant failure than any other single cause. AMR is mediated by antibodies recognizing antigens expressed by the graft, and antibodies generated against major histocompatibility complex (MHC) mismatches are especially problematic. Most research directed
Congjing An et al.
Cancer cell international, 22(1), 184-184 (2022-05-14)
Breast cancer (BC) is one of the most prevalent malignancies among women globally. Emerging evidence indicates that long non-coding RNAs (lncRNAs) are associated with BC carcinogenesis. In the current study, we explored the mechanism by which LINC00662 regulates BC. Quantitative
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