Merck
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MAB1576

Sigma-Aldrich

Anti-Muscarinic Acetylcholine Receptor m4 Antibody, clone 17F10.2

purified antibody, clone 17F10.2, Chemicon®

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eCl@ss:
32160702
NACRES:
NA.41

生物来源

mouse

质量水平

100

抗体形式

purified antibody

antibody product type

primary antibodies

克隆

17F10.2, monoclonal

species reactivity

mouse, monkey, rat

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

同位素/亚型

IgG1

NCBI登记号

NM_000741.2

UniProt登记号

P08173

运输

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Chrm4(12672)
rat ... Chrm4(25111)
rhesus monkey ... Chrm4(574195)

特异性

m4 muscarinic acetylcholine receptor. No reactivity with the other subtypes.

免疫原

m4 receptor i3 loop (human), fused to GST

应用

Immunohistochemistry (rat and monkey)

Western blot (rat, mouse, human): ECL 4-10 μg/mL

Immunoprecipitation (rat)

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
This Anti-Muscarinic Acetylcholine Receptor m4 Antibody, clone 17F10.2 is validated for use in IC, IP, WB for the detection of Muscarinic Acetylcholine Receptor m4.

外形

Liquid in 0.02M Phosphate buffer, 0.25 M NaCl, pH=7.6 with 0.1% sodium azide.

储存及稳定性

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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Lack of specificity of commercially available antisera: better specifications needed.
Pradidarcheep, W; Labruyere, WT; Dabhoiwala, NF; Lamers, WH
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society null
Taketsugu Hayashi et al.
Cell reports, 30(13), 4433-4444 (2020-04-03)
The ability to infer others' mental states is essential to social interactions. This ability, critically evaluated by testing whether one attributes false beliefs (FBs) to others, has been considered to be uniquely hominid and to accompany the activation of a
Eleanor D Muise et al.
Data in brief, 10, 482-486 (2017-01-06)
The data presented in this article are related to the research article entitled "Distribution of muscarinic acetylcholine receptor subtypes in the murine small intestine" (E.D. Muise, N. Gandotra, J.J. Tackett, M.C. Bamdad, R.A. Cowles, 2016) [1]. We recently demonstrated that
Franziska Richter et al.
PloS one, 14(12), e0226080-e0226080 (2019-12-06)
In early-onset generalized torsion dystonia, caused by a GAG deletion in TOR1A (DYT1), enhanced striatal cholinergic activity has been suggested to be critically involved. Previous studies have shown increased acetylcholine levels in the striatum of DYT1 knock-in (KI) mice. Ex
Sinead M Clancy et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 27(24), 6388-6399 (2007-06-15)
Many inhibitory neurotransmitters in the brain activate Kir3 channels by stimulating pertussis toxin (PTX)-sensitive G-protein-coupled receptors. Here, we investigated the regulation of native muscarinic receptors and Kir3 channels expressed in NGF-differentiated PC12 cells, which are similar to sympathetic neurons. Quantitative
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