MAB16985
抗MCAM抗体，克隆P1H12
clone P1H12, Chemicon®, from mouse
别名:
CD146 antigen, Cell surface glycoprotein P1H12, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen, melanoma adhesion molecule, melanoma cell adhesion molecule
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
P1H12, monoclonal
Application:
ELISA
flow cytometry
immunocytochemistry
immunohistochemistry
immunoprecipitation (IP)
western blot
flow cytometry
immunocytochemistry
immunohistochemistry
immunoprecipitation (IP)
western blot
Species reactivity:
mouse, canine, human
Citations:
28
Technique(s):
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Uniprot accession no.:
产品名称
抗MCAM抗体，克隆P1H12, clone P1H12, Chemicon®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
P1H12, monoclonal
species reactivity
mouse, canine, human
should not react with
rat
packaging
antibody small pack of 25 μg
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
suitability
not suitable for immunohistochemistry (Paraffin)
NCBI accession no.
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MCAM(4162)
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Analysis Note
对照
HUVEC 细胞。
HUVEC 细胞。
通过蛋白质印迹对 HUVEC 裂解液进行了常规评估。
蛋白质印迹分析：
该批次以 1：500 的稀释度在 10μg HUVEC 裂解液中检测到 MCAM。
蛋白质印迹分析：
该批次以 1：500 的稀释度在 10μg HUVEC 裂解液中检测到 MCAM。
Application
免疫细胞化学：
1-10 μg/mL 先前批次的抗体用于免疫细胞化学。在 EDTA 或胰蛋白酶悬浮的内皮细胞中效果最佳。
免疫组化：
1-10 µg/mL。4% PFA，室温下30min或4°C下<2h。采用1%BSA/0.2% tween20/PBS封闭30min。非常适合固定或未固定的冷冻组织。
免疫沉淀
1-10 μg/mL 先前批次用于免疫沉淀。
ELISA：
1-10 μg/mL 先前批次用于 ELISA。
FACS 分析：
1-10 μg/mL 先前批次用于 FACS。
最佳工作稀释度必须由最终用户确定。
1-10 μg/mL 先前批次的抗体用于免疫细胞化学。在 EDTA 或胰蛋白酶悬浮的内皮细胞中效果最佳。
免疫组化：
1-10 µg/mL。4% PFA，室温下30min或4°C下<2h。采用1%BSA/0.2% tween20/PBS封闭30min。非常适合固定或未固定的冷冻组织。
免疫沉淀
1-10 μg/mL 先前批次用于免疫沉淀。
ELISA：
1-10 μg/mL 先前批次用于 ELISA。
FACS 分析：
1-10 μg/mL 先前批次用于 FACS。
最佳工作稀释度必须由最终用户确定。
抗 MCAM 抗体，克隆 P1H12 检测 MCAM 的水平，&经发表 & 验证可用于 ELISA、FC、IC、IH、IP & WB。
Biochem/physiol Actions
检测人的循环内皮细胞。 预期与狗 & 小鼠发生交叉反应。
General description
MUC18 抗原（CD146）是免疫球蛋白超家族的成员，是一种 110-120 kDa 的固有膜糖蛋白，存在于内皮细胞、骨髓成纤维细胞和各种黑色素瘤的表面。MUC18 已被用作人类黑色素瘤肿瘤进展的标志物，因为 MUC18 在这些肿瘤中的表达与不良预后和转移性疾病的发展密切相关。另外，许多人 T、B 和骨髓性白血病细胞系似乎表达 MUC18。与 N-CAM 和相关分子的紧密结构关系表明，MUC18 也可能是一种发育受调节的细胞粘附分子（黑色素瘤粘附分子或 MCAM）。
110kda
Immunogen
用 HUVEC 免疫小鼠。
Other Notes
浓度：请参考批次特异性浓缩物的分析证书。
Physical form
在含 0.02 M PB 和 0.25 M NaCl，pH = 7.6 以及 0.1％ 叠氮化钠的缓冲液中的纯化的小鼠单克隆 IgG1。
形式：纯化的
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
David M Neskey et al.
Journal of experimental & clinical cancer research : CR, 27, 61-61 (2008-11-06)
Endostatin and anastellin, fragments of collagen type XVIII and fibronectin, respectively, belong to a family of endogenous inhibitors of angiogenesis which inhibit tumor growth and metastasis in a number of mouse models of human cancer. The mechanism of action of
Ivan Depasquale et al.
Cancer cell international, 6, 9-9 (2006-04-01)
Lovastatin and other statins may reduce the development of melanomas. The effects on melanoma cells and their ability to enhance angiogenesis in a co-culture system presented an opportunity to assess whether Lovastatin act on melanoma cells, HUVEC or both types
Feng Li et al.
Bone, 47(3), 546-555 (2010-06-24)
Currently, there are conflicting data in literature regarding contribution of bone marrow stromal cells (BMSCs) to bone formation when the cells are systemically delivered in recipient animals. To understand if BMSCs contribute to bone cell phenotype and bone formation in
Yong Wook Park et al.
The American journal of pathology, 165(6), 2087-2098 (2004-12-08)
Thrombospondin 2 (TSP2), a matricellular protein with a primary role in modulating cell-matrix interactions, has been implicated in tissue repair and foreign body responses. Here we show that TSP2 has regulatory function in the chronic inflammatory lesions of rheumatoid arthritis.
Keiich Hirono et al.
Journal of the American College of Cardiology, 48(6), 1257-1264 (2006-09-19)
This study investigated patients with acute Kawasaki disease (KD) to validate myeloid-related protein (MRP)-8/MRP-14 as a marker of disease activity and severity of coronary artery lesion development. Both MRP-8 and -14, which are S100-proteins secreted by activated neutrophils and monocytes
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