MAB1951
Anti-Integrin beta 1 (CD29) Antibody
CHEMICON®, mouse monoclonal, P4G11
别名:
CD29
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关于此项目
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
P4G11, monoclonal
Species reactivity:
monkey, rabbit, human
Application:
ELISA
cell culture | mammalian
flow cytometry
immunocytochemistry
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
immunoprecipitation (IP)
cell culture | mammalian
flow cytometry
immunocytochemistry
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
immunoprecipitation (IP)
Technique(s):
ELISA: suitable
cell culture | mammalian: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
cell culture | mammalian: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
Citations:
15
Uniprot accession no.:
产品名称
抗整合素β 1抗体，克隆P4G11, clone P4G11, Chemicon®, from mouse
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
P4G11, monoclonal
species reactivity
monkey, rabbit, human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
cell culture | mammalian: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ITGB1(3688)
Analysis Note
对照
扁桃体，人皮肤组织
扁桃体，人皮肤组织
Application
免疫组化：用于丙酮或轻度多聚甲醛固定组织（2-2.5%PFA，短时间）冷冻和4%PFA石蜡包埋{Qin et al 2003 1：500，处理的柠檬酸盐HEIR}
结合所需的Ca++。Ca++必须用于阻断、洗涤和抗体培养缓冲液中。
不推荐传统福尔马林固定。
免疫细胞化学：2 ug/ml（只要Ca2+存在，可用于许多细胞系）
免疫沉淀：5 μg/400 μL裂解物（0.5%triton PBS加Ca++）
FACS：在Ca++存在下，每10e6个细胞2-5μg。
细胞刺激：2-10μ g/ml/10e6细胞。结合缓冲液必须含有1mM Ca++。
ELISA
最佳工作稀释度必须由最终用户确定。
结合所需的Ca++。Ca++必须用于阻断、洗涤和抗体培养缓冲液中。
不推荐传统福尔马林固定。
免疫细胞化学：2 ug/ml（只要Ca2+存在，可用于许多细胞系）
免疫沉淀：5 μg/400 μL裂解物（0.5%triton PBS加Ca++）
FACS：在Ca++存在下，每10e6个细胞2-5μg。
细胞刺激：2-10μ g/ml/10e6细胞。结合缓冲液必须含有1mM Ca++。
ELISA
最佳工作稀释度必须由最终用户确定。
抗整联蛋白β1抗体，克隆P4G11是抗整联蛋白β1的抗体，用于ELISA、FC、IC、IH、IH（P）、IP、FUNC、CULT。
研究子类别
整联蛋白
整联蛋白
研究类别
细胞结构
细胞结构
Biochem/physiol Actions
与整联蛋白β1亚基上的钙依赖性表位反应。当与β1结合时，P4G11激活依赖性结合于细胞外基质底物的β1。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
Immunogen
人IL-1β活化内皮细胞
Other Notes
浓度：请参考批次特异性浓缩物的分析证书。
Physical form
形式：纯化
纯化的蛋白 A
蛋白A纯化的免疫球蛋白，溶于0.02 M PBS，0.25 M NaCl，pH值7.6和0.1%NaN3中。
Preparation Note
自发运之日起，在 2–8°C 条件下可稳定保存 1 年等分以避免反复冻融。为了最大程度地回收产品，在融化后和取下盖子之前，将原始样品瓶进行离心。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Migrating glioma cells activate the PI3-K pathway and display decreased susceptibility to apoptosis.
Anna M Joy et al.
Journal of cell science, 116(Pt 21), 4409-4417 (2003-09-18)
Glioma cells that migrate out of the main tumor mass into normal brain tissue contribute to the failure of most gliomas to respond to treatment. Treatments that target migratory glioma cells may enhance the therapeutic response. Multiple lines of evidence
Jesus Vega-Lugo et al.
The Journal of cell biology, 221(7) (2022-05-14)
Colocalization analysis of multicolor microscopy images is a cornerstone approach in cell biology. It provides information on the localization of molecules within subcellular compartments and allows the interrogation of known molecular interactions in their cellular context. However, almost all colocalization
Convergence of eicosanoid and integrin biology: 12-lipoxygenase seeks a partner.
Tang, K; Cai, Y; Joshi, S; Tovar, E; Tucker, SC; Maddipati, KR; Crissman, JD; Repaskey, WT; Honn, KV
Molecular Cancer null
C Radel et al.
American journal of physiology. Heart and circulatory physiology, 288(2), H936-H945 (2004-10-09)
To identify the role of caveolin-1 in integrin mechanotransduction, we exposed bovine aortic endothelial cells to 10 dyn/cm2 of laminar shear stress. Caveolin-1 was acutely and transiently phosphorylated with shear, occurring downstream of beta1-integrin activation as the beta1-integrin blocking antibody
Francesca Ricciardiello et al.
Cell death & disease, 9(3), 377-377 (2018-03-09)
Cancer aberrant N- and O-linked protein glycosylation, frequently resulting from an augmented flux through the Hexosamine Biosynthetic Pathway (HBP), play different roles in tumor progression. However, the low specificity and toxicity of the existing HBP inhibitors prevented their use for
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