产品名称
抗整合素α2β1抗体,克隆BHA2.1, clone BHA2.1, Chemicon®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
BHA2.1, monoclonal
species reactivity
human, canine, pig
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ITGA2(3673)
Analysis Note
对照
皮肤,CD4& CD8阳性T细胞
皮肤,CD4& CD8阳性T细胞
Application
抗整联蛋白α2β1抗体,克隆BHA2.1是一种抗整联蛋白α2β1的抗体,用于FC、IH、IH(P)&IP。
流式细胞术: 10 μg/mL
免疫沉淀: 从10E6细胞当量的裂解物中进行VLA-2整联蛋白的免疫沉淀需要2-5μg BHA2.1
冷冻组织切片的免疫组化: 5μg/mL
石蜡切片中的免疫组化: 1:50。 使用80%乙醇中的0.5%H2O2阻断内源性过氧化物酶30分钟,非特异性反应使用PBS中的NGS 1:5,室温下20分钟。 Bouins ′固定液已成功使用。 培养时间,条件:24小时;4°C;预处理:0.1%胰蛋白酶,30 min,37°C。 细胞膜附近的胞浆内染色。
可能由于所涉及表位的构象特性,无法通过蛋白质印迹检测α2整联蛋白亚基。BHA2.1的表位取决于α2整联蛋白亚基的(I)结构域的存在,并且不结合缺少I结构域的VLA-2变体。
功能阻断: BHA2.1抑制VLA-2介导的细胞对胶原和层粘连蛋白的粘连;通常可以在10 μg/mL的最终浓度下实现完全抑制。
最终工作稀释度必须由最终用户确定。
免疫沉淀: 从10E6细胞当量的裂解物中进行VLA-2整联蛋白的免疫沉淀需要2-5μg BHA2.1
冷冻组织切片的免疫组化: 5μg/mL
石蜡切片中的免疫组化: 1:50。 使用80%乙醇中的0.5%H2O2阻断内源性过氧化物酶30分钟,非特异性反应使用PBS中的NGS 1:5,室温下20分钟。 Bouins ′固定液已成功使用。 培养时间,条件:24小时;4°C;预处理:0.1%胰蛋白酶,30 min,37°C。 细胞膜附近的胞浆内染色。
可能由于所涉及表位的构象特性,无法通过蛋白质印迹检测α2整联蛋白亚基。BHA2.1的表位取决于α2整联蛋白亚基的(I)结构域的存在,并且不结合缺少I结构域的VLA-2变体。
功能阻断: BHA2.1抑制VLA-2介导的细胞对胶原和层粘连蛋白的粘连;通常可以在10 μg/mL的最终浓度下实现完全抑制。
最终工作稀释度必须由最终用户确定。
研究子类别
整联蛋白
整联蛋白
研究类别
细胞结构
细胞结构
Biochem/physiol Actions
与胶原受体VLA-2(α-2β-1)整联蛋白复合物反应。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
Other Notes
浓度:请参考批次特异性浓缩物的分析证书。
Physical form
形式:纯化
纯化的蛋白 A
通过蛋白 A 层析纯化的免疫球蛋白。在0.02 M PB pH值7.6、0.25 M NaCl,0.1%叠氮化钠中以液体形式存在。
Preparation Note
自发运之日起,在 2–8°C 条件下可稳定保存 1 年等分以避免反复冻融。为了最大程度地回收产品,在融化后和取下盖子之前,将原始样品瓶进行离心。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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