MAB2029
Anti-CSPG4 Antibody
CHEMICON®, mouse monoclonal, 9.2.27
别名:
Anti-CSPG4A, Anti-MCSP, Anti-MCSPG, Anti-MEL-CSPG, Anti-MSK16, Anti-NG2
产品名称
硫酸软骨素蛋白聚糖抗体，克隆9.2.27, clone 9.2.27, Chemicon®, from mouse
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
9.2.27, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CSPG4(1464)
Application
可使用这种硫酸软骨素蛋白聚糖抗体（克隆9.2.27）检测硫酸软骨素蛋白聚糖，该抗体经验证可用于FC、IF & IP。
培养细胞的免疫荧光显微镜检查（Bumoi et al., 1984; Schrappe et al., 1992）。此外，Legg J et al (2003) Development 130:6049-6063 {3% PFA3固定的冷冻切片｝。
免疫电子显微镜检查(Bumoi et al., 1984)
免疫沉淀：1μg/10E6个细胞(Bumoi et al., 1984; Morgan et al., 1981)
FACS分析：1-2μg/10E6个细胞
黑色素瘤细胞扩散的功能性阻滞（Morgan et al., 1981），裸鼠体内的神经胶质瘤细胞增殖3和肿瘤生长（Morgan et al., 1981; Schrappe et al., 1992）。
最佳工作稀释度必须由最终用户进行确定。
免疫电子显微镜检查(Bumoi et al., 1984)
免疫沉淀：1μg/10E6个细胞(Bumoi et al., 1984; Morgan et al., 1981)
FACS分析：1-2μg/10E6个细胞
黑色素瘤细胞扩散的功能性阻滞（Morgan et al., 1981），裸鼠体内的神经胶质瘤细胞增殖3和肿瘤生长（Morgan et al., 1981; Schrappe et al., 1992）。
最佳工作稀释度必须由最终用户进行确定。
研究子类别
ECM 蛋白
ECM 蛋白
研究类别
细胞结构
细胞结构
Biochem/physiol Actions
看识别250 kDa的成熟硫酸软骨素蛋白聚糖核心糖蛋白以及210、220和240 kDa的前体多肽（Bumoi et al., 1984）。可与人黑色素瘤、神经胶质瘤和增殖的脑内皮细胞反应。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
Immunogen
耗尽纤连蛋白并由小扁豆凝集素-Sepharose结合的人M14黑色素瘤细胞提取物（Morgan et al., 1981）。
Other Notes
浓度：请参考批次特异性浓缩物的检验报告。
Physical form
形式：纯化
纯化的免疫球蛋白，溶于含0.25M NaCl和0.1%叠氮化钠的0.02M PBS（pH 7.6）中。
Preparation Note
未稀释的等分试样在2-8°C可保存6个月。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Henrietta M Nielsen et al.
Acta neuropathologica communications, 1, 7-7 (2013-11-21)
Neuron Glial 2 (NG2) cells are glial cells known to serve as oligodendrocyte progenitors as well as modulators of the neuronal network. Altered NG2 cell morphology and up-regulation as well as increased shedding of the proteoglycan NG2 expressed on the
Nina Schultz et al.
Aging cell, 17(3), e12728-e12728 (2018-02-18)
The population of brain pericytes, a cell type important for vessel stability and blood brain barrier function, has recently been shown altered in patients with Alzheimer's disease (AD). The underlying reason for this alteration is not fully understood, but progressive
Sabrina Cattaruzza et al.
Angiogenesis, 16(2), 309-327 (2012-11-06)
Sprouting of angiogenic perivascular cells is thought to be highly dependent upon autocrine and paracrine growth factor stimulation. Accordingly, we report that corneal angiogenesis induced by ectopic FGF implantation is strongly impaired in NG2/CSPG4 proteoglycan (PG) null mice known to
Molly C McCloskey et al.
Journal of visualized experiments : JoVE, (203) (2024-01-29)
The microSiM (µSiM) is a membrane-based culture platform for modeling the blood-brain barrier (BBB). Unlike conventional membrane-based platforms, the µSiM provides experimentalists with new capabilities, including live cell imaging, unhindered paracrine signaling between 'blood' and 'brain' chambers, and the ability to
Nina Schultz et al.
Neurobiology of aging, 69, 94-101 (2018-06-05)
Islet amyloid polypeptide (IAPP) forms toxic aggregates in the brain of patients with Alzheimer's disease (AD). Whether IAPP also affects the retina in these patients is still unknown. Levels of IAPP in soluble and insoluble homogenate fractions of retina and
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