产品名称
抗酪氨酸羟化酶抗体,克隆LNC1, ascites fluid, clone LNC1, Chemicon®
biological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
LNC1, monoclonal
species reactivity
zebrafish, mouse, rat, vole, human, frog, lizard, monkey, chicken
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
General description
Immunogen
Application
之前一个批次的1:200-1:400稀释液被用于IH。4% PFA固定的冷冻切片;4% PFA固定的石蜡切片1:100(Barrachina, M. et al., 2003)。针对石蜡切片,Barrachina曾报道过采用柠檬酸微波微波抗原回收成功进行过染色,但其他方法也应该可以使用。
免疫沉淀:
之前一个批次的该抗体被用于IP(Perez, 2002)。
终端用户需要对最佳的工作稀释比和实验方法进行确定。
神经递质 & 受体
神经元 & 胶质标记
神经科学
Biochem/physiol Actions
Physical form
Preparation Note
Analysis Note
人脑组织,提取自用Forskolin处理(40 nM,30 min)的3T3细胞。
Western blot:
该批次1:1000的稀释液对10 μg 小鼠大脑裂解液中的酪氨酸羟化酶进行了检测。
Other Notes
Legal Information
Disclaimer
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存储类别
12 - Non Combustible Liquids
实验方案
Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.
商品
Antibodies combine with specific antigens to generate an exclusive antibody-antigen complex. Learn about the nature of this bond and its use as a molecular tag for research.
Learn differences in monoclonal vs polyclonal antibodies including how antibodies are generated, clone numbers, and antibody formats.
Explore the basics of working with antibodies including technical information on structure, classes, and normal immunoglobulin ranges.
相关内容
抗体的质量决定了整个免疫检测的成败, 是在选择抗体时首选的考虑因素。基于Sigma®、Chemicon®、Upstate®、Mil l ipore®、 Calbiochem®和Novagen®等子品牌的强强联合,默克在创新的 免疫原设计、免疫、挑选、筛选和验证等方面有着数十年的经验, 创造出许多被广泛使用的抗体,如4G10,Neun,组蛋白修饰等抗 体都是相关领域的“金标”抗体。 我们的技术和科研背景使默克成为高质量抗体的主要设计者和生产 者,而不仅仅是经销商。毋庸置疑,我们的每个小瓶中的抗体都包 含着大量的科学研究成果。
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| MAB318 | 08436037123320 |