产品名称
抗BrdU抗体,克隆IIB5, clone IIB5, Chemicon®, from mouse
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
IIB5, monoclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
isotype
IgG1
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Analysis Note
对照
掺入BrdU后,全部含DNA的物种
掺入BrdU后,全部含DNA的物种
Application
免疫组化:1:100-1:200在进行酸/蛋白酶处理的冷冻和石蜡包埋的组织上。参见:
http://www.protocol-online.org/prot/Immunology/Immunohistochemistry.各种方案的示例。
流式细胞术: 1:100-1:200;需要酸性甲醇处理。
最佳工作稀释度必须由最终用户进行确定。
为了使抗体起作用,需要进行预处理以暴露DNA(在r/t条件下,1 mg/mL胃蛋白酶在0.01 M HCL中30分钟)
http://www.protocol-online.org/prot/Immunology/Immunohistochemistry.各种方案的示例。
流式细胞术: 1:100-1:200;需要酸性甲醇处理。
最佳工作稀释度必须由最终用户进行确定。
为了使抗体起作用,需要进行预处理以暴露DNA(在r/t条件下,1 mg/mL胃蛋白酶在0.01 M HCL中30分钟)
抗BrdU抗体(克隆IIB5)是用于检测溴脱氧尿苷(也称为BrdU)的小鼠单克隆抗体,&已在FC、ICC、IHC(P)中进行了验证。
研究子类别
细胞周期,DNA 复制&修复
细胞周期,DNA 复制&修复
研究类别
表观遗传学&核功能
表观遗传学&核功能
Biochem/physiol Actions
与溴脱氧尿苷发生特异性反应。 当掺入核DNA时,它还可以识别BrdU。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
掺入DNA的溴脱氧尿苷(BrdU)的免疫细胞化学检测是研究正常细胞和赘生性细胞的细胞动力学的有力工具。用胸苷类似物BrdU体外或体内标记肿瘤细胞,然后用特异性抗BrdU单克隆抗体检测掺入的BrdU是定量DNA合成程度的准确全面的方法。BrdU被掺入S期细胞的新合成DNA中,因此可以为S期细胞比例提供估计值。还可以通过双变量BrdU/DNA流式细胞术分析获得动态增殖信息(例如S期转运率和潜在的倍增时间)。
Immunogen
小鼠骨髓瘤细胞与来自用与牛血清白蛋白结合的BrdU腹膜内免疫的小鼠的脾细胞的融合。
Other Notes
浓度:请参考批次特异性浓缩物的分析证书。
Physical form
形式:纯化
纯化的蛋白 A
蛋白A纯化的小鼠免疫球蛋白溶于含0.09% 叠氮化钠的PBS中。
Preparation Note
自发运之日起在-20°C可保存1年。等分以避免反复冻融。为了最大程度地回收产品,在融化后和取下盖子之前,将原始样品瓶进行离心。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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