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Merck
CN

MAB339

抗GABA A受体α1 链抗体(NT,克隆BD24)

clone BD24, Chemicon®, from mouse

别名:

Anti-DEE19, Anti-ECA4, Anti-EIEE19, Anti-EJM, Anti-EJM5

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
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产品名称

抗GABA A受体α1 链抗体(NT,克隆BD24), clone BD24, Chemicon®, from mouse

isotype

IgG1

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

BD24, monoclonal

species reactivity

human, pig, avian, bovine

should not react with

feline, rat

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

Gene Information

human ... GABRA1(2554)

Application

免疫组化:10-20 μg/mL *请参阅以下方案。

蛋白质印迹:20 μg/mL

免疫沉淀

最佳工作稀释度必须由最终用户确定。

MAB339应用说明

免疫组化

1)应使用新鲜组织(人) (死后3-6 小时)。组织应浸入2%多聚甲醛/0.1%戊二醛。制备50 mm切片并在-15°C下保存在冷冻保护液中。

2)切片应当在合适小瓶中悬浮培养。

3) 以100 mmol/L Tris-HCl、50 mmol/L NaCl(pH 7.4 )含3%H2O2(v/v)和10% 甲醇的(缓冲液A)在室温下封闭内源性过氧化物酶20分钟。

4)在缓冲液A中清洗切片。将切片与含5% 胎牛血清(v/v)和0.1-0.5% Triton X-100 (v/v)的缓冲液A一起培养。

5)在缓冲液A中清洗切片。将切片与MAB339(稀释10-20 mg/mL)在含5% 胎牛血清(v/v)和0.1-0.5% Triton X-100 (v/v)的缓冲液A中一起在+4°C 下培养12-36小时。

6)在缓冲液A中清洗切片。

7)以标准二抗检测系统((PAP、ABC等)检测。

8)在缓冲液A中清洗切片。

9)将切片安装在涂覆铬明矾的载玻片上,干燥,脱水并盖上盖玻片。
抗-GABA A受体α1链抗体(N末端,克隆BD24)是针对GABA A受体α1链的抗体,用于IH、IP&WB。
研究子类别
神经递质 & 受体
研究类别
神经科学

Biochem/physiol Actions

与牛和人GABAA受体的α1链反应,但不与大鼠反应。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

Immunogen

来自牛皮质的纯化GABA/苯二氮受体
表位:N端

Other Notes

浓度:请参考批次特异性浓缩物的检验报告。

Physical form

形式:纯化
液体。缓冲液=0.02M磷酸盐缓冲液、0.25M NaCl和0.1%叠氮化钠。

Preparation Note

冻干物料可在+2-8°C保存长达12个月。重新配制后,以未稀释等分试样在 -20°C可冷冻保存长达 6个月。应避免反复冻/融循环。

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Raunak Sinha et al.
Cell, 168(3), 413-426 (2017-01-28)
The fovea is a specialized region of the retina that dominates the visual perception of primates by providing high chromatic and spatial acuity. While the foveal and peripheral retina share a similar core circuit architecture, they exhibit profound functional differences
Simone M Brändle et al.
Proceedings of the National Academy of Sciences of the United States of America, 118(9) (2021-02-24)
Encephalitis associated with antibodies against the neuronal gamma-aminobutyric acid A receptor (GABAA-R) is a rare form of autoimmune encephalitis. The pathogenesis is still unknown but autoimmune mechanisms were surmised. Here we identified a strongly expanded B cell clone in the
Ivan Milenkovic et al.
Brain structure & function, 223(3), 1501-1518 (2017-11-24)
The function, regulation and cellular distribution of GABAA receptor subunits have been extensively documented in the adult rodent brain and are linked to numerous neurological disorders. However, there is a surprising lack of knowledge on the cellular (sub-) distribution of
Wen-Yi Lo et al.
Neurochemical research, 39(6), 1088-1103 (2013-11-12)
GABAA receptors, the major mediators of fast inhibitory neuronal transmission, are heteropentameric glycoproteins assembled from a panel of subunits, usually including α and β subunits with or without a γ2 subunit. The α1β2γ2 receptor is the most abundant GABAA receptor
Vaishali S Janve et al.
Annals of neurology, 79(5), 806-825 (2016-03-08)
The Epi4K Consortium recently identified 4 de novo mutations in the γ-aminobutyric acid type A (GABAA ) receptor β3 subunit gene GABRB3 and 1 in the β1 subunit gene GABRB1 in children with one of the epileptic encephalopathies (EEs) Lennox-Gastaut

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