产品名称
抗GABA A受体α1 链抗体(NT,克隆BD24), clone BD24, Chemicon®, from mouse
isotype
IgG1
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
BD24, monoclonal
species reactivity
human, pig, avian, bovine
should not react with
feline, rat
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... GABRA1(2554)
Application
免疫组化:10-20 μg/mL *请参阅以下方案。
蛋白质印迹:20 μg/mL
免疫沉淀
最佳工作稀释度必须由最终用户确定。
MAB339应用说明
免疫组化
1)应使用新鲜组织(人) (死后3-6 小时)。组织应浸入2%多聚甲醛/0.1%戊二醛。制备50 mm切片并在-15°C下保存在冷冻保护液中。
2)切片应当在合适小瓶中悬浮培养。
3) 以100 mmol/L Tris-HCl、50 mmol/L NaCl(pH 7.4 )含3%H2O2(v/v)和10% 甲醇的(缓冲液A)在室温下封闭内源性过氧化物酶20分钟。
4)在缓冲液A中清洗切片。将切片与含5% 胎牛血清(v/v)和0.1-0.5% Triton X-100 (v/v)的缓冲液A一起培养。
5)在缓冲液A中清洗切片。将切片与MAB339(稀释10-20 mg/mL)在含5% 胎牛血清(v/v)和0.1-0.5% Triton X-100 (v/v)的缓冲液A中一起在+4°C 下培养12-36小时。
6)在缓冲液A中清洗切片。
7)以标准二抗检测系统((PAP、ABC等)检测。
8)在缓冲液A中清洗切片。
9)将切片安装在涂覆铬明矾的载玻片上,干燥,脱水并盖上盖玻片。
蛋白质印迹:20 μg/mL
免疫沉淀
最佳工作稀释度必须由最终用户确定。
MAB339应用说明
免疫组化
1)应使用新鲜组织(人) (死后3-6 小时)。组织应浸入2%多聚甲醛/0.1%戊二醛。制备50 mm切片并在-15°C下保存在冷冻保护液中。
2)切片应当在合适小瓶中悬浮培养。
3) 以100 mmol/L Tris-HCl、50 mmol/L NaCl(pH 7.4 )含3%H2O2(v/v)和10% 甲醇的(缓冲液A)在室温下封闭内源性过氧化物酶20分钟。
4)在缓冲液A中清洗切片。将切片与含5% 胎牛血清(v/v)和0.1-0.5% Triton X-100 (v/v)的缓冲液A一起培养。
5)在缓冲液A中清洗切片。将切片与MAB339(稀释10-20 mg/mL)在含5% 胎牛血清(v/v)和0.1-0.5% Triton X-100 (v/v)的缓冲液A中一起在+4°C 下培养12-36小时。
6)在缓冲液A中清洗切片。
7)以标准二抗检测系统((PAP、ABC等)检测。
8)在缓冲液A中清洗切片。
9)将切片安装在涂覆铬明矾的载玻片上,干燥,脱水并盖上盖玻片。
抗-GABA A受体α1链抗体(N末端,克隆BD24)是针对GABA A受体α1链的抗体,用于IH、IP&WB。
研究子类别
神经递质 & 受体
神经递质 & 受体
研究类别
神经科学
神经科学
Biochem/physiol Actions
与牛和人GABAA受体的α1链反应,但不与大鼠反应。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
Immunogen
来自牛皮质的纯化GABA/苯二氮受体
表位:N端
Other Notes
浓度:请参考批次特异性浓缩物的检验报告。
Physical form
形式:纯化
液体。缓冲液=0.02M磷酸盐缓冲液、0.25M NaCl和0.1%叠氮化钠。
Preparation Note
冻干物料可在+2-8°C保存长达12个月。重新配制后,以未稀释等分试样在 -20°C可冷冻保存长达 6个月。应避免反复冻/融循环。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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