产品名称
抗异染色质蛋白-1β抗体，克隆1MOD-1A9, ascites fluid, clone 1MOD-1A9, Chemicon®
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
1MOD-1A9, monoclonal
species reactivity
mouse, human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CBX1(10951)
Analysis Note
对照
HeLa、293或NIH/3T3细胞裂解物
HeLa、293或NIH/3T3细胞裂解物
Application
使用经ELISA、ICC、IHC、IP、WB验证的抗异染色质蛋白-1β抗体，克隆1MOD-1A9（小鼠单克隆抗体）检测异染色质蛋白-1β（又称HP1b）。
蛋白质印迹：1:500-1:5,000
免疫荧光：1:500-1:5,000
免疫组化：1:500-1:5,000
免疫沉淀：1:500 - 1:5,000
ELISA：1:500-1:5,000
最佳工作稀释度必须由最终用户确定。
免疫荧光：1:500-1:5,000
免疫组化：1:500-1:5,000
免疫沉淀：1:500 - 1:5,000
ELISA：1:500-1:5,000
最佳工作稀释度必须由最终用户确定。
Biochem/physiol Actions
与异染色质蛋白-1b（HP1b）反应。
General description
28kda
染色质组装因子1（CAF-1）是一种多亚基蛋白复合物，包含三个称为p150，p60和p48的多肽亚基。CAF-1是一种核小体组装因子，在DNA复制过程中将新合成的和乙酰化的组蛋白H3/H4沉积到新生的染色质中。
异染色质的特征是密集卷曲的染色质，通常在S期后期复制，基因密度低，并且包含大块重复DNA，而DNA修饰试剂相对无法获得。在S期后期，p150与异染色质相关蛋白1（HP1α，HP1β和HP1γ）直接缔合。随着细胞准备有丝分裂，CAF-1 p150和一些HP1逐渐从异染色质上解离，这与组蛋白H3的磷酸化相吻合。由于组蛋白H3被去磷酸化，HP1蛋白在有丝分裂结束时与染色质重新结合。
异染色质的特征是密集卷曲的染色质，通常在S期后期复制，基因密度低，并且包含大块重复DNA，而DNA修饰试剂相对无法获得。在S期后期，p150与异染色质相关蛋白1（HP1α，HP1β和HP1γ）直接缔合。随着细胞准备有丝分裂，CAF-1 p150和一些HP1逐渐从异染色质上解离，这与组蛋白H3的磷酸化相吻合。由于组蛋白H3被去磷酸化，HP1蛋白在有丝分裂结束时与染色质重新结合。
Immunogen
重组小鼠HP1β。
Other Notes
浓度：请参考批次特异性浓缩物的分析证书。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Sarah Herberg et al.
Scientific reports, 5, 14236-14236 (2015-09-22)
Transposable elements in the genome are generally silenced in differentiated somatic cells. However, increasing evidence indicates that some of them are actively transcribed in early embryos and the proper regulation of retrotransposon expression is essential for normal development. Although their
Epigenetic regulation of myogenic gene expression by heterochromatin protein 1 alpha.
Sdek, P; Oyama, K; Angelis, E; Chan, SS; Schenke-Layland, K; MacLellan, WR
Testing null
Kyoko Hiragami-Hamada et al.
Nature communications, 7, 11310-11310 (2016-04-20)
Histone H3 trimethylation of lysine 9 (H3K9me3) and proteins of the heterochromatin protein 1 (HP1) family are hallmarks of heterochromatin, a state of compacted DNA essential for genome stability and long-term transcriptional silencing. The mechanisms by which H3K9me3 and HP1
Erik Müllers et al.
Aging cell, 16(3), 575-584 (2017-03-28)
In response to DNA damage, a cell can be forced to permanently exit the cell cycle and become senescent. Senescence provides an early barrier against tumor development by preventing proliferation of cells with damaged DNA. By studying single cells, we
Annika Baude et al.
Nucleic acids research, 44(5), 2214-2226 (2016-01-02)
We have recently identified lens epithelium-derived growth factor (LEDGF/p75, also known as PSIP1) as a component of the homologous recombination DNA repair machinery. Through its Pro-Trp-Trp-Pro (PWWP) domain, LEDGF/p75 binds to histone marks associated with active transcription and promotes DNA
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