MAB3510
抗-BrdU抗体，克隆BU-1
culture supernatant, clone BU-1, Chemicon®
别名:
BrdU
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
BU-1, monoclonal
Technique(s):
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
Application:
flow cytometry
immunocytochemistry
immunohistochemistry
immunocytochemistry
immunohistochemistry
Citations:
31
产品名称
抗-BrdU抗体，克隆BU-1, culture supernatant, clone BU-1, Chemicon®
biological source
mouse
antibody form
culture supernatant
antibody product type
primary antibodies
clone
BU-1, monoclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
isotype
IgG2a
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Analysis Note
对照
Brdu处理细胞
Brdu处理细胞
Application
使用经流式细胞术、免疫细胞化学和免疫组织化学验证的小鼠单克隆抗BrdU抗体（克隆BU-1）检测溴脱氧尿苷（BrdU）。
尽管最初报道染色核DNA BrdU掺入乙醇固定细胞中而无酸或碱变性，但该性质后来归因于存在于所用支原体污染的BU-1杂交瘤培养物上清液中的DNase活性（Gonchoroff，N.J.，et al.（1986）.J. Immunol. Methods.93(1):97-101; Greipp, P.R., et al. (1985).Am. J. Hematol.20（3）:289-292）。在使用本产品进行免疫染色之前，请在样品制备过程中加入适当的DNA变性步骤。
研究子类别
细胞周期、DNA复制&修复
细胞周期、DNA复制&修复
研究类别
表观遗传学&核功能
表观遗传学&核功能
Biochem/physiol Actions
克隆BU-1与溴脱氧尿苷特异性结合，与碘尿苷的交叉反应性为10%（10%）。克隆BU-1不会与氟脱氧尿苷或任何内源性细胞成分（例如胸苷或尿苷）发生交叉反应。
Disclaimer
除非我们的目录或产品随附的其他公司文件中另有说明，否则我们的产品预期仅用于研究用途，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人类或动物的任何类型的消费或应用。
General description
溴脱氧尿苷（BrdU）是胸苷类似物，当外源添加时，在DNA合成过程中会掺入DNA中。抗溴脱氧尿苷单克隆抗体用于标记已掺入BrdU的增殖细胞。 与使用放射性胸苷掺入来鉴定正在复制的细胞相比，该免疫学检测方案具有多个优势。标记和检测可以在同一天进行，而不是像氚标记的细胞放射自显影所要求的那样等待几天，并且消除了使用多个标本获得最佳曝光时间的必要性。此外，通过流式细胞术分析进行抗溴脱氧尿苷染色可以同时评估多个参数。
Immunogen
溴脱氧尿苷
表位：BrdU
Other Notes
浓度：关于批次特定浓度请参见检验报告。
Physical form
未纯化
来自灌注系统的杂交瘤培养物上清液，在装瓶前通过0.2微米膜过滤。产品包含20% FBS和环丙沙星，终浓度为10 μg/mL。本产品中可能存在支原体风险。采用适当措施防止细胞系感染/污染。
Preparation Note
自收到之日起在-20°C可稳定保存1年。分装保存以避免反复冻融。为了最大程度地回收产品，需在融化后和取下盖子之前将原始样品管进行离心。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Jessica Dragotto et al.
Journal of applied genetics, 60(3-4), 357-365 (2019-09-06)
The Npc1nih/nih-null model and the Npc1nmf164/nmf164 hypomorph models of Niemann-Pick C1 (NPC1) disease show defects in olfaction. We have tested the effects of the life-prolonging treatment hydroxypropyl-beta-cyclodextrin (HPBCD) on olfaction and neural stem cell numbers when delivered either systemically or
Leonid A Serebryannyy et al.
Journal of cell science, 129(18), 3412-3425 (2016-08-10)
Actin is abundant in the nucleus and it is clear that nuclear actin has important functions. However, mystery surrounds the absence of classical actin filaments in the nucleus. To address this question, we investigated how polymerizing nuclear actin into persistent
Miwako Kobayashi et al.
Molecular brain, 7, 12-12 (2014-02-18)
We have previously identified BRINP (BMP/RA-inducible neural-specific protein-1, 2, 3) family genes that possess the ability to suppress cell cycle progression in neural stem cells. Of the three family members, BRINP1 is the most highly expressed in various brain regions
Differential involvement of estrogen receptor alpha and estrogen receptor beta in the healing promoting effect of estrogen in human keratinocytes.
Merlo, S; Frasca, G; Canonico, PL; Sortino, MA
The Journal of Endocrinology null
Hypoxia-ischemia induces an endogenous reparative response by local neural progenitors in the postnatal mouse telencephalon.
Dizon, M; Szele, F; Kessler, JA
Developmental Neuroscience null
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