MAB3864
抗-DNA/组蛋白H1抗体
Chemicon®, from mouse
别名:
Anti-H1.4, Anti-H1E, Anti-H1F4, Anti-H1s-4, Anti-HIST1H1E, Anti-RMNS, Anti-dJ221C16.5
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
monoclonal
Application:
ELISA, FACS, ICC, IHC
Citations:
39
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
monoclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... H1-1(3024)
Immunogen
人Raji细胞核
Application
免疫细胞化学：5-10 μg/mL用于2%多聚甲醛固定的细胞（室温10分钟）
免疫组织化学：5-10 μg/mL用于多聚甲醛或B5固定的组织切片。
流式细胞术：5-10 μg/mL，使用2%多聚甲醛固定的细胞。
ELISA、直接和间接
最佳工作稀释度必须由最终用户确定。
染色模式：
观察到核染色。细胞核浓染。核仁无染色。
免疫组织化学：5-10 μg/mL用于多聚甲醛或B5固定的组织切片。
流式细胞术：5-10 μg/mL，使用2%多聚甲醛固定的细胞。
ELISA、直接和间接
最佳工作稀释度必须由最终用户确定。
染色模式：
观察到核染色。细胞核浓染。核仁无染色。
抗DNA/组蛋白H1抗体是用于检测DNA/组蛋白H1的高质量小鼠单克隆抗体，&已在ELISA、FC、ICC & IHC中进行了验证。
研究子类别
组蛋白
组蛋白
研究类别
表观遗传学&核功能
表观遗传学&核功能
Biochem/physiol Actions
与DNA/组蛋白1反应。该抗体可用于染色所有物种的细胞核并鉴定坏死。通过免疫组织化学，抗体显示细胞核密集染色，未观察到核仁染色。
Physical form
形式：纯化
蛋白A纯化的培养物上清液。 溶于PBS中的液体
Preparation Note
自接收之日起，以未稀释的等分试样在-20°C下保存长达6个月。应避免反复冻/融循环。
Other Notes
浓度：关于批次特定浓度请参见检验报告。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
除非我们的目录或产品随附的其他公司文件中另有说明，否则我们的产品预期仅用于研究用途，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人类或动物的任何类型的消费或应用。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Neila Álvarez de Haro et al.
Fish & shellfish immunology, 119, 209-219 (2021-08-27)
Neutrophils release chromatin extracellular traps (ETs) as part of the fish innate immune response to counter the threats posed by microbial pathogens. However, relatively little attention has been paid to this phenomenon in many commercially farmed species, despite the importance
Nicole de Buhr et al.
Cellular microbiology, 19(2) (2016-07-28)
Streptococcus suis is an important meningitis-causing pathogen in pigs and humans. Neutrophil extracellular traps (NETs) have been identified as host defense mechanism against different pathogens. Here, NETs were detected in the cerebrospinal fluid (CSF) of S. suis-infected piglets despite the presence
Nicole de Buhr et al.
Frontiers in microbiology, 9, 966-966 (2018-06-06)
Granulocytes mediate the first line of defense against infectious diseases in humans as well as animals and they are well known as multitasking cells. They can mediate antimicrobial activity by different strategies depending on the pathogen they encounter. Besides phagocytosis
Verena Peek et al.
Pharmaceuticals (Basel, Switzerland), 14(6) (2021-07-03)
High mobility group box (HMGB)1 action contributes to late phases of sepsis, but the effects of increased endogenous plasma HMGB1 levels on brain cells during inflammation are unclear. Here, we aimed to further investigate the role of HMGB1 in the
Simona Grasso et al.
Thrombosis research, 161, 36-42 (2017-11-28)
The circulating zymogen form of Factor VII activating protease (FSAP) can be activated by histones and nucleosomes in vivo. These cell-death-associated nuclear factors are also actively extruded into the extracellular space by neutrophils through a process called neutrophil extracellular trap
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