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Merck
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MAB4155

抗-BCRP1抗体，克隆5D3

clone 5D3, Chemicon®, from mouse

别名:

ABC transporter, ATP-binding cassette transporter G2, ATP-binding cassette, sub-family G (WHITE), member 2, ATP-binding cassette, sub-family G, member 2, Breast cancer resistance protein, CD338 antigen, Mitoxantrone resistance-associated protein, Placent

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

主要文件

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产品名称

抗-BCRP1抗体，克隆5D3, clone 5D3, Chemicon®, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5D3, monoclonal

species reactivity

rat, human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable

input

sample type neural stem cell(s)
sample type hematopoietic stem cell(s)

isotype

IgG2bκ

NCBI accession no.

NM_004827.2

UniProt accession no.

Q9UNQ0

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... BCRP1(644079)

相关类别

Analysis Note

对照
MCF-7细胞
通过蛋白质印迹法对L6裂解物进行常规评估。

蛋白质印迹分析：
该批次以1:500稀释度在10 μg L6裂解物中检测到BCRP1。

Application

使用经验证可用于FC、IC、IF、FUNC & WB的抗BCRP1抗体（克隆5D3）检测BCRP1。
免疫细胞化学：
细胞培养物在室温下用4% PFA固定5-15分钟。 用含0.3% Triton X-100的PBS中的10% NGS、0.1% BSA封闭（Ginis et al.，2004）。

流式细胞术:
使用先前批次的1:100-1:1000稀释液。

抑制试验:
一个先前的批次被用于抑制ABCG2（Watanabe, K. et al., (2004.FEBS Letters 565:6-10）。也参见Sarkadi，B.，et al.，2004 FEBS 567:116-120。 Sarkadi报告5D3的检测和抑制强烈依赖于ABCG2的实际构象。
用含0.3% triton X-100的PBS中的10% NGS、0.1% BSA封闭（Ginis，2004）。

最佳工作稀释度必须由最终使用者进行确定。
研究子类别
神经干细胞

造血干细胞
研究类别
干细胞研究

Biochem/physiol Actions

特异性识别人ABCG2的外部表位。据报道与小鼠干细胞系反应（Ginis，et al.，2004），但是此时不支持小鼠反应性。

Disclaimer

除非我们的目录或产品随附的其他公司文件中另有说明，否则我们的产品预期仅用于研究用途，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人类或动物的任何类型的消费或应用。

General description

72 kDa
乳腺癌抗性蛋白（BCRP1），也称为胎盘特异性ATP结合盒(ABC)蛋白(ABCP)或ABC G亚家族成员2（ABCG2）或米托蒽醌抗性蛋白(MXR)，是药物转运蛋白的ATP结合盒超家族的成员，被认为与人类肿瘤疾病的多药耐药性有关。ABCG2在耐药性乳腺癌、结肠癌和胃癌以及纤维肉瘤癌细胞系和急性髓性白血病(AML)患者的原始细胞中表达。表达ABCG2蛋白的正常组织包括胎盘合体滋养细胞、乳腺导管和小叶、大肠和结肠的上皮、静脉和毛细血管上皮以及肝脏的胆小管膜。多项研究表明，ABCG2也在不同组织的干细胞中表达，并且可以用作干细胞标志物。人们认为ABCG2负责非常原始的造血和非造血细胞群体（所谓的SP（侧群）子集）流出Hoechst 33342荧光染料的能力。半定量RT-PCR结果表明，与人、恒河猴和小鼠造血组织中的非SP细胞相比，ABCG2 mRNA在SP细胞中的表达水平更高，但成红细胞和NK细胞除外。在人骨髓中，与分化程度更高的CD34+CD38+祖细胞相比，在表型定义的原始CD34+CD38-造血细胞群体中ABCG2 mRNA表达更高。Bcrp1 mRNA也在小鼠骨骼肌的SP细胞、神经干细胞和祖细胞以及巢蛋白阳性胰岛衍生的祖细胞中表达。通过反转录病毒介导的基因转移过表达人ABCG2基因赋予小鼠骨髓细胞SP表型。Bcrp1 mRNA在小鼠胚胎干细胞的SP和非SP组分中表达。这表明Bcrp1蛋白水平可能并不总是由mRNA水平反映出来，或者SP表型也涉及干细胞的其他特征，例如细胞周期状态或其他转运蛋白的表达。

Immunogen

表达人ABCG2的3T3细胞

Other Notes

浓度：关于批次特定浓度请参见检验报告。

Physical form

形式：纯化
纯化的小鼠单克隆IgG2bκ液体，溶于含0.1%叠氮化钠的PBS（pH 7.4）的缓冲液中。
纯化蛋白A

Preparation Note

自接收之日起，在2-8ºC下可稳定保存1年。 请勿冷冻。

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Wenji Zhang et al.
The Journal of biological chemistry, 294(52), 20222-20232 (2019-11-14)
ATP-binding cassette (ABC) subfamily G member 2 (ABCG2) belongs to the ABC transporter superfamily and has been implicated in multidrug resistance of cancers. Although the structure and function of ABCG2 have been extensively studied, little is known about its biogenesis
A Khammanivong et al.
Veterinary and comparative oncology, 14(3), e113-e125 (2014-08-13)
Canine hemangiosarcoma is a rapidly progressive disease that is poorly responsive to conventional chemotherapy. Despite numerous attempts to advance treatment options and improve outcomes, drug resistance remains a hurdle to successful therapy. To address this problem, we used recently characterized
Comparative proteomics reveals human pluripotent stem cell-derived limbal epithelial stem cells are similar to native ocular surface epithelial cells.
Mikhailova, A; Jylha, A; Rieck, J; Nattinen, J; Ilmarinen, T; Vereb, Z; Aapola et al.
Scientific Reports null
Christophe Antczak et al.
Assay and drug development technologies, 12(1), 28-42 (2013-09-03)
ABCG2 is a member of the ATP-binding cassette (ABC) family of transporters, the overexpression of which has been implicated in resistance to various chemotherapeutic agents. Though a number of cell-based assays to screen for inhibitors have been reported, they do
Andrei A Kramerov et al.
Molecular vision, 21, 1357-1367 (2016-01-21)
To examine the expression of putative limbal epithelial stem cell (LESC) markers and wound healing rates in primary healthy and diabetic human limbal epithelial cells (LECs) cultured on different substrata. Primary limbal epithelial cells were isolated from human autopsy corneas
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