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Merck
CN

MAB4304

Sigma-Aldrich

抗-阶段特异性胚胎抗原-4抗体，克隆MC-813-70

clone MC-813-70, Chemicon®, from mouse

别名:

SSEA-4

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关于此项目

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

主要文件

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生物来源

mouse

质量水平

100

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

MC-813-70, monoclonal

种属反应性

human, mouse

制造商/商品名称

Chemicon®

技术

ELISA: suitable
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable

输入

sample type: mouse embryonic stem cell(s)
sample type: human embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

同位素/亚型

IgG3

运输

wet ice

靶向翻译后修饰

unmodified

相关类别

一般描述

通常用于鉴定未分化的人类胚胎干细胞（hESC）的细胞表面标志物最初被表征为mESC、小鼠胚胎性癌(EC)或人类EC细胞的标志物。阶段特异性胚胎抗原3（SSEA-3）和SSEA-4被广泛用作定义人和小鼠ESC的细胞表面标志物。SSEA-3和SSEA-4在未分化的hESC上表达，但在未分化的mESC上不表达。

免疫原

人胚胎性癌细胞系2102Ep

应用

免疫组织化学： 该抗体的一个先前批次已被用于IH。

免疫荧光：该抗体的一个先前批次已被用于IF。

ELISA： 该抗体的一个先前批次已被用于ELISA。

流式细胞术: 建议起始范围为10-20 µg/mL。

最佳工作稀释度必须由最终用户进行确定。
抗阶段特异性胚胎抗原-4抗体（克隆MC-813-70）是一种抗阶段特异性胚胎抗原-4的抗体，用于FC、ELISA、IF、IH。
研究子类别
多能性&早期分化
研究类别
干细胞研究

生化/生理作用

该抗体与人四癌干细胞(EC)、人胚胎生殖细胞(EG)和人胚胎干细胞(ES)表面表达的阶段特异性胚胎抗原-4（SSEA-4）反应。 未分化的鼠EC、ES和EG细胞没有明显的免疫反应性。 人EC细胞分化后，SSEA-4的表达下调。 相反，鼠EC和ES细胞的分化可能伴随着SSEA-4表达的增加。

外形

形式：纯化
纯化的小鼠单克隆IgG3液体，溶于含0.02 M PBS、0.25 M NaCl（pH 7.6）和0.1%叠氮化钠的缓冲液中
纯化蛋白A

制备说明

自接收之日起1年内保持稳定。

分析说明

对照
多能人胚胎干(ES)细胞

其他说明

浓度：关于批次特定浓度请参见检验报告。

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

除非我们的目录或产品随附的其他公司文件中另有说明，否则我们的产品预期仅用于研究用途，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人类或动物的任何类型的消费或应用。

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储存分类代码

10 - Combustible liquids

WGK

WGK 2

分析证书(COA)

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James A Byrne et al.
PloS one, 4(9), e7118-e7118 (2009-09-24)
The derivation of induced pluripotent stem cells (iPSCs) provides new possibilities for basic research and novel cell-based therapies. Limitations, however, include our current lack of understanding regarding the underlying mechanisms and the inefficiency of reprogramming. Here, we report identification and
Hidehito Saito et al.
Stem cells international, 2016, 8394960-8394960 (2016-04-09)
Induced pluripotent stem cells (iPSCs) derived from somatic cells of patients hold great promise for autologous cell therapies. One of the possible applications of iPSCs is to use them as a cell source for producing autologous lymphocytes for cell-based therapy
Lei Kang et al.
Stem cells and development, 25(7), 556-568 (2016-02-18)
Podocalyxin-like protein (PODXL) is a member of CD34 family proteins. It is the protein that carries many post-translational epitopes responsible for various pluripotent surface markers including TRA-1-60, TRA-1-81, GCTM2, GP200, and mAb84. However, PODXL has not attracted the attention of
Epigenetic characterization of the FMR1 gene and aberrant neurodevelopment in human induced pluripotent stem cell models of fragile X syndrome.
Sheridan, SD; Theriault, KM; Reis, SA; Zhou, F; Madison, JM; Daheron, L; Loring, JF; Haggarty, SJ
Testing null
Hui Zhu et al.
PloS one, 11(3), e0151836-e0151836 (2016-03-19)
Human embryonic stem cells (hESCs) are derived from the inner cell mass (ICM) of blastocyst staged embryos. Spare blastocyst staged embryos were obtained by in vitro fertilization (IVF) and donated for research purposes. hESCs carrying specific mutations can be used
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