产品名称
抗视网膜色素上皮65抗体, clone 401.8B11.3D9, Chemicon®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
401.8B11.3D9, monoclonal
species reactivity
bovine, human, Xenopus, mouse
packaging
antibody small pack of 25 μg
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... RPE65(6121)
Physical form
形式：纯化
纯化的免疫球蛋白 溶于PBS中的液体。不含防腐剂。
纯化的蛋白 A
Analysis Note
对照
眼睛
眼睛
Application
抗视网膜色素上皮细胞65抗体可检测视网膜色素上皮细胞65的水平 & 已发表 & 并经过验证可用于ELISA、IH、IP、 & WB。
研究子类别
感官 & PNS
感官 & PNS
研究类别
神经科学
神经科学
蛋白质印迹：使用ECL在牛RPE膜上进行反应的稀释度为1：5,000-1：20,000。建议的稀释缓冲液是含有10％小牛血清、0.25％T-20、1M D-葡萄糖和10％甘油的TBS。建议的封闭缓冲液是含有2％BSA和0.5％Tween 20的TBS。优先选择的凝胶百分比为10％。
在冷冻组织切片上进行免疫组织化学分析：1：250-1：500
免疫沉淀：500 μL反应体积中20 μg抗体。
免疫亲和纯化
ELISA
最佳工作稀释液必须由最终用户进行确定。
在冷冻组织切片上进行免疫组织化学分析：1：250-1：500
免疫沉淀：500 μL反应体积中20 μg抗体。
免疫亲和纯化
ELISA
最佳工作稀释液必须由最终用户进行确定。
Biochem/physiol Actions
可与视网膜色素上皮细胞65（RPE65）反应。在牛RPE膜上，该抗体可识别分子量约为65 kDa的蛋白质。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
~ 65 kDa
Immunogen
牛RPE微粒体膜。
Other Notes
浓度：请参考批次特异性浓缩物的分析证书。
Preparation Note
自收到之日起在2–8°C可稳定保存1年。避免反复冻融。为了最大程度地回收产品，在融化后和取下盖子之前，将原始样品瓶进行离心。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Mu?iz, A; Greene, WA; Plamper, ML; Choi, JH; Johnson, AJ; Tsin, AT; Wang, HC
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