产品名称
抗-5-甲基胞嘧啶抗体，克隆33D3, clone 33D3, from mouse
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
33D3, monoclonal
species reactivity
human, mouse
technique(s)
Southern blotting: suitable
dot blot: suitable
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
methylated DNA immunoprecipitation (MeDIP): suitable
isotype
IgG1λ
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Analysis Note
对照
甲基化和未甲基化BRCA1
甲基化和未甲基化BRCA1
通过斑点印迹法在甲基化和未甲基化BRCA1中进行评价。
斑点印迹分析： 1 µg/mL该抗体在甲基化和非甲基化BRCA1中检测到甲基胞嘧啶。
斑点印迹分析： 1 µg/mL该抗体在甲基化和非甲基化BRCA1中检测到甲基胞嘧啶。
Application
IF
Habib, M., et al. (1999).Exp Cell Res. 249:46-53
IHC-P
Hernadez-Blazquez, F.J., et al. (2000).Gut.47:689-693
Flow Cytometry
Reynaud, C.C., et al. (1992).Cancer Lett.61(3):255-262
MeDIP
Keshet, I., et al. (2006).Nat Genet.38(2):149-153
Habib, M., et al. (1999).Exp Cell Res. 249:46-53
IHC-P
Hernadez-Blazquez, F.J., et al. (2000).Gut.47:689-693
Flow Cytometry
Reynaud, C.C., et al. (1992).Cancer Lett.61(3):255-262
MeDIP
Keshet, I., et al. (2006).Nat Genet.38(2):149-153
使用在DB、IF、SB、IHC(P)、流式细胞术、MeDIP中验证的抗5-甲基胞嘧啶抗体，克隆33D3（小鼠单克隆抗体）检测5-甲基胞嘧啶，也称为甲基胞嘧啶、甲基胞苷。
研究子类别
染色质生物学
染色质生物学
研究类别
表观遗传学&核功能
表观遗传学&核功能
Biochem/physiol Actions
该抗体可识别5-甲基胞嘧啶，但不能识别未甲基化的胞嘧啶。
预计会与多种物种发生反应。
Disclaimer
除非我们的目录或产品随附的其他公司文件中另有说明，否则我们的产品预期仅用于研究用途，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人类或动物的任何类型的消费或应用。
General description
5-甲基胞嘧啶是在高等真核生物的DNA中发现的修饰碱基。已知DNA的甲基化参与基因表达的控制，并由一类称为DNA甲基转移酶(DNMT’)的酶介导。适当的胞嘧啶甲基化对于哺乳动物细胞的正常发育和维持至关重要。DNA甲基化模式的改变通常与癌症有关，与正常组织的DNA相比，肿瘤DNA被低甲基化。
检测甲基化胞嘧啶
Immunogen
卵清蛋白结合5-甲基胞嘧啶
Other Notes
替代：MABE527
Physical form
形式：纯化
纯化的小鼠单克隆IgG1λ，溶于含PBS和0.05%叠氮化钠的缓冲液中。
蛋白A
Preparation Note
自接收之日起，在-20°C下可稳定保存1年。
处理建议: 收到后，在取下瓶盖之前，将小瓶离心并轻轻混合溶液。分装至微量离心管中，并储存于-20°C。避免反复冻融循环，否则可能损坏IgG并影响产品性能。
处理建议: 收到后，在取下瓶盖之前，将小瓶离心并轻轻混合溶液。分装至微量离心管中，并储存于-20°C。避免反复冻融循环，否则可能损坏IgG并影响产品性能。
未找到合适的产品？
试试我们的产品选型工具.
存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Elena M Pugacheva et al.
Epigenetics & chromatin, 9(1), 35-35 (2016-09-03)
A common aberration in cancer is the activation of germline-specific proteins. The DNA-binding proteins among them could generate novel chromatin states, not found in normal cells. The germline-specific transcription factor BORIS/CTCFL, a paralog of chromatin architecture protein CTCF, is often
Olga A Efimova et al.
Oncotarget, 8(51), 88294-88307 (2017-11-29)
We performed immunofluorescent analysis of DNA hydroxymethylation and methylation in human testicular spermatogenic cells from azoospermic patients and ejaculated spermatozoa from sperm donors and patients from infertile couples. In contrast to methylation which was present throughout spermatogenesis, hydroxymethylation was either
Yize Zhang et al.
Scientific reports, 7, 43158-43158 (2017-02-23)
In vertebrates, DNA methyltransferase 3 (Dnmt3) homologues are responsible for de novo DNA methylation and play important roles in germ cell development. In the present study, four dnmt3 genes, dnmt3aa, dnmt3ab, dnmt3ba and dnmt3bb.1, were identified in ricefield eels. Real-time
Alain Cuna et al.
American journal of respiratory cell and molecular biology, 53(1), 60-73 (2014-11-12)
DNA methylation, a major epigenetic mechanism, may regulate coordinated expression of multiple genes at specific time points during alveolar septation in lung development. The objective of this study was to identify genes regulated by methylation during normal septation in mice
Yidan Liu et al.
Epigenetics, 12(7), 551-560 (2017-04-14)
The 5-methylcytosine (5mC) modification regulates multiple cellular processes and is faithfully maintained following DNA replication. In addition to DNA methyltransferase (DNMT) family proteins, ubiquitin-like PHD and ring finger domain-containing protein 1 (UHRF1) plays an important role in the maintenance of
相关内容
New Products: Antibodies, Assays, Small Molecules, Inhibitors, and Proteins
Signaling Product Guide: Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持