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Merck
CN

MABE50

抗-磷酸化表位SR蛋白抗体,克隆1H4

clone 1H4, from mouse

别名:

Ser-Arg-rich proteins

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
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产品名称

抗-磷酸化表位SR蛋白抗体,克隆1H4, clone 1H4, from mouse

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1H4, monoclonal

species reactivity

rat

species reactivity (predicted by homology)

human (based on 100% sequence homology), mouse (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

Analysis Note

对照
L6 plus胰岛素细胞裂解物
通过蛋白质印迹法在L6加胰岛素细胞裂解物中进行评估。

蛋白质印迹分析:0.025 µg/mL的该抗体在10 µg L6加胰岛素细胞裂解物中检测到SR蛋白。

Application

使用经WB、ICC、IP验证的抗磷酸表位SR蛋白抗体,克隆1H4(小鼠单克隆抗体)检测磷酸表位SR蛋白(也称为富含Ser-Arg的蛋白)。
免疫细胞化学分析:一个代表性批次被独立实验室用于IC(Fukuhara,T.,et al.(2006).

免疫沉淀分析:一个代表性批次被独立实验室用于进行IP(Buratti,E.,et al.(2007).
研究子类别
RNA代谢&结合蛋白
研究类别
表观遗传学&核功能

Disclaimer

除非我们的目录或产品随附的其他公司文件中另有说明,否则我们的产品预期仅用于研究用途,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人类或动物的任何类型的消费或应用。

General description

富含Ser-Arg(SR)的蛋白质构成功能和结构上保守的磷蛋白家族,并且是替代性和组成性pre-mRNA剪接的必需组成部分。SR蛋白的特征在于其模块化组成由两个目标结构域组成:用于确定DNA结合特异性的N末端RNA识别基序(RRM)和RS结构域(富含精氨酸和丝氨酸的C末端结构域,充当SR蛋白的穿梭和定位导向器以及剪接激活结构域。它们参与前mRNA剪接的各个方面和剪接体组装,包括;剪接位点的识别和分配,以及选择性剪接调控的操纵。
观测分子量约75、55、40、30和20 kDa。
该抗体可识别由75、55、40、30、20 kDa蛋白组成的SR蛋白家族。

Immunogen

含有非洲爪蟾SR蛋白的纯化卵母细胞核。

Other Notes

浓度:关于批次特定浓度请参见检验报告。

Physical form

形式:纯化
纯化的小鼠单克隆IgG1κ,溶于含0.1 M Tris-甘氨酸(pH 7.4)、150 mM NaCl和0.05%叠氮化钠的缓冲液中。
纯化蛋白G

Preparation Note

自接收之日起,在2-8°C下可稳定保存1年。

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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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