产品名称
抗-PARG抗体，克隆D8B10, clone D8B10, from mouse
shipped in
wet ice
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
D8B10, monoclonal
species reactivity
human
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PARG(8505)
Other Notes
浓度：关于批次特定浓度请参见检验报告。
Physical form
形式：纯化
纯化的小鼠单克隆IgG2aκ，溶于含0.1 M Tris-甘氨酸（pH 7.4）、150 mM NaCl和0.05％叠氮化钠的缓冲液中。
蛋白G
General description
聚（ADP-核糖）糖水解酶(PARG)是一种对聚（ADP-核糖）(PARP)具有内切和外切糖苷酶活性的酶，可迅速降解PARP以释放大量游离ADP-核糖。PARG参与了几个细胞过程，包括：凋亡、DNA修复、细胞周期进程、细胞存活和细胞分化。在凋亡过程中，PARG被胱天蛋白酶-3裂解，表明PARG活性在此过程中受到调节。尽管由一个基因编码，但PARG以不同的亚型存在于不同的细胞定位中: 亚型1（111 kDa）存在于细胞核中，亚型2（102 kDa）存在于细胞质中，亚型3（99 kDa）存在于线粒体中。研究表明，PARG亚型1在精子染色质质量和随后的胚胎存活中起关键作用。
观测分子量〜102 kDa。 在111和99 kDa的一些裂解物中可观察到其他亚型
Analysis Note
对照
Jurkat细胞裂解物
Jurkat细胞裂解物
通过蛋白质印迹法在Jurkat细胞裂解物中进行评价。
蛋白质印迹分析：0.5 µg/mL的该抗体在10 µg Jurkat细胞裂解物上检测到PARG。
蛋白质印迹分析：0.5 µg/mL的该抗体在10 µg Jurkat细胞裂解物上检测到PARG。
Application
抗PARG抗体，克隆D8B10是用于WB&IP的抗PARG抗体。
研究子类别
激素&受体
染色质生物学
激素&受体
染色质生物学
研究类别
信号传导
表观遗传学&核功能
信号传导
表观遗传学&核功能
Disclaimer
除非我们的目录或产品随附的其他公司文件中另有说明，否则我们的产品预期仅用于研究用途，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人类或动物的任何类型的消费或应用。
Immunogen
对应于人PARG的组氨酸标记的重组蛋白。
表位：未知
Preparation Note
自接收之日起，在2-8°C下可稳定保存1年。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Yajie Zhang et al.
Nature methods, 10(10), 981-984 (2013-08-21)
Poly(ADP-ribosyl)ation is catalyzed by a family of enzymes known as PARPs. We describe a method to characterize the human aspartic acid- and glutamic acid-ADP-ribosylated proteome. We identified 1,048 ADP-ribosylation sites on 340 proteins involved in a wide array of nuclear
Jean-Christophe Amé et al.
Methods in molecular biology (Clifton, N.J.), 1608, 395-413 (2017-07-12)
The purification of Poly(ADP-ribose) glycohydrolase (PARG) from overexpressing bacteria Escherichia coli is described here to a fast and reproducible one chromatographic step protocol. After cell lysis, GST-PARG-fusion proteins from the crude extract are affinity purified by a Glutathione 4B Sepharose
Kaede Tsuda et al.
Cancer reports (Hoboken, N.J.), 6(2), e1709-e1709 (2022-09-03)
Poly(ADP-ribose) glycohydrolase (PARG) is a key enzyme in poly(ADP-ribose) (PAR) metabolism and a potential anticancer target. Many drug candidates have been developed to inhibit its enzymatic activity. Additionally, PDD00017273 is an effective and selective inhibitor of PARG at the first
Xiaoxing Feng et al.
Molecular cancer, 11, 48-48 (2012-07-31)
Cell death induced by poly(ADP-ribose) (PAR) and mediated by apoptosis-inducing factor (AIF) is well-characterized in models of ischemic tissue injury, but their roles in cancer cell death after chemotherapy are less understood. Here we investigated the roles of PAR and
Francesco Morra et al.
Journal of experimental & clinical cancer research : CR, 41(1), 245-245 (2022-08-14)
Treatment with PARP inhibitors (PARPi) is primarily effective against high-grade serous ovarian cancers (HGSOC) with BRCA1/2 mutations or other deficiencies in homologous recombination (HR) repair mechanisms. However, resistance to PARPi frequently develops, mostly as a result of BRCA1/2 reversion mutations.
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