MABT336
抗-TKS5抗体，克隆13H6.3
clone 13H6.3, from mouse
别名:
Adapter protein TKS5, Five SH3 domain-containing protein, SH3 multiple domains protein 1, Tyrosine kinase substrate with five SH3 domains, SH3 and PX domain-containing protein 2A
登录 查看组织和合同定价。
选择尺寸
关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
13H6.3, monoclonal
Application:
immunohistochemistry
western blot
western blot
Species reactivity:
human
Citations:
6
Technique(s):
immunohistochemistry: suitable (paraffin)
western blot: suitable
western blot: suitable
Uniprot accession no.:
产品名称
抗-TKS5抗体，克隆13H6.3, clone 13H6.3, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
13H6.3, monoclonal
species reactivity
human
packaging
antibody small pack of 25 μg
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Quality Level
Gene Information
human ... SH3PXD2A(9644)
Analysis Note
通过蛋白质印迹法在SCC61和SK-MEL28细胞裂解物中进行评估。
蛋白质印迹分析：0.5 µg/mL的该抗体在10 µg SCC61和SK-MEL28细胞裂解物中检测到TKS5。
蛋白质印迹分析：0.5 µg/mL的该抗体在10 µg SCC61和SK-MEL28细胞裂解物中检测到TKS5。
Application
免疫组织化学分析：一个代表性批次以1:50稀释度在人乳腺癌组织中检测到TKS5。
抗TKS5，克隆13H6.3，目录号MABT336，是一种检测TKS5的小鼠单克隆抗体，已被检验用于免疫组织化学（石蜡）和蛋白质印迹。
研究类别
细胞结构
细胞结构
Biochem/physiol Actions
克隆13H6.3可检测人细胞中含有SH3和PX结构域的蛋白2A（衔接蛋白TKS5）。它靶向C末端一半有60个氨基酸的表位。
Disclaimer
除非我们的目录或产品随附的其他公司文件中另有说明，否则我们的产品预期仅用于研究用途，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人类或动物的任何类型的消费或应用。
General description
含有SH3和PX结构域的蛋白2A（UniProt：Q5TCZ1；又称衔接蛋白TKS5、含5个SH3结构域蛋白、SH3多结构域蛋白1、含5个SH3结构域的酪氨酸激酶底物）由人的SH3PXD2A（又称FISH、KIAA0418、SH3MD1、TKS5）基因编码（基因ID：9644）。衔接蛋白TKS5是一种脚手架蛋白，是足体形成、细胞外基质降解以及某些癌细胞侵袭所必需的。已经描述了通过选择性剪接产生的TKS5的三种亚型。TKS5是正常细胞中的一种细胞质蛋白，但它位于SRC转化细胞中的足体中。在几种癌细胞系中发现，特别是浸润性乳腺癌和黑色素瘤。TKS5包含一个PX（phx同源性）域和五个SH3域。PX域是足体定位所必需的，因为它具有结合磷酸肌醇（例如PtdIns3P和PtdIns（3，4）P2）以及较小程度的PtdIns5P和PtdIns（3，5）P2的能力。它与ADAM12、ADAM15和ADAM19的结合是由第五个SH3结构域介导的。PX结构域与第三个SH3结构域的分子内相互作用将蛋白质维持在细胞质中，磷酸化破坏了这种相互作用，导致蛋白质从细胞质重新分布到膜周围区域。
观测分子量〜160 kDa；计算分子量为125.29 kDa。在某些裂解物中可以观察到未鉴定的条带。
Immunogen
GST/His标记的重组片段，对应于人SH3和含PX域的蛋白2A（衔接蛋白TKS5）C末端一半的60个氨基酸。
Other Notes
浓度：请参考特定批次的数据表。
Physical form
形式：纯化
纯化小鼠单克隆抗体IgG1，溶于含0.1 M Tris-甘氨酸（pH 7.4）、150 mM NaCl和0.05%叠氮化钠的缓冲液中。
纯化蛋白G
Preparation Note
自接收之日起，在2-8°C下可稳定保存1年。
未找到合适的产品？
试试我们的产品选型工具.
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Matthew Baik et al.
MethodsX, 6, 718-726 (2019-04-24)
Invadopodia, cancer cell protrusive structures with associated proteolytic activity, provide cancer cells with the ability to remodel the extracellular matrix. Invadopodia facilitate invasive migration and their formation correlates with cancer cell invasiveness and metastatic potential. The unambiguous identification of invadopodia
Louisiane Perrin et al.
Communications biology, 5(1), 758-758 (2022-08-02)
Invasive and non-invasive cancer cells can invade together during cooperative invasion. However, the events leading to it, role of the epithelial-mesenchymal transition and the consequences this may have on metastasis are unknown. In this study, we demonstrate that the isogenic
Battuya Bayarmagnai et al.
Journal of cell science, 132(20) (2019-09-20)
The process of tumor cell invasion and metastasis includes assembly of invadopodia, protrusions capable of degrading the extracellular matrix (ECM). The effect of cell cycle progression on invadopodia has not been elucidated. In this study, by using invadopodia and cell
Chenxi Tian et al.
Cancer research, 80(7), 1461-1474 (2020-02-08)
The prognosis for pancreatic ductal adenocarcinoma (PDAC) remains poor despite decades of effort. The abundant extracellular matrix (ECM) in PDAC comprises a major fraction of the tumor mass and plays various roles in promoting resistance to therapies. However, nonselective depletion
Juan Liu et al.
Nature metabolism, 4(12), 1830-1846 (2022-12-20)
The glycolytic enzyme lactate dehydrogenase A (LDHA) is frequently overexpressed in cancer, which promotes glycolysis and cancer. The oncogenic effect of LDHA has been attributed to its glycolytic enzyme activity. Here we report an unexpected noncanonical oncogenic mechanism of LDHA;
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持