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Merck
CN

MHA000P2N

Wallerstein Nutrient Broth

ampoule capacity 2 mL, ampoule filling volume 2 mL, ready-to-use, pack of 50 tests, for bacteria, for yeasts, for molds

别名:

WL Nutrient broth, WLNB, Wallerstein Laboratory Nutrient Broth

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UNSPSC Code:
41106225
NACRES:
NA.74
eCl@ss:
32160801

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产品名称

Wallerstein Nutrient Broth, ampoule capacity 2 mL, ampoule filling volume 2 mL, ready-to-use, pack of 50 tests, for bacteria, for yeasts, for molds

sterility

sterile

form

liquid

shelf life

12 mo.

feature

ready-to-use

packaging

pack of 50 tests

ampoule capacity

2 mL

ampoule filling volume

2 mL

color

slightly opalescent green-blue

input

sample type bottled water
sample type beer
sample type sport drink
sample type cider
sample type food(s)
sample type beverage(s)
sample type wine
sample type cosmetics
sample type soft drink
sample type beverage(s)

pH

5.5±0.2 (25.0 °C)

Quality Level

100

application(s)

environmental
food and beverages
industrial qc
microbiology

compatibility

for use with MF-method
for use with Microfil®
for use with MicropreSure®
for use with Milliflex®

shipped in

wet ice

storage temp.

2-8°C

suitability

bacteria
molds
yeasts

相关类别

Analysis Note

Bacterial colonies can appear non-pigmented to white, with a smooth or rough texture. Yeast appear as creamy, white larger colonies
QC organisms
L. fermentum (ATCC 9338)

S. cerevisiae (ATCC 9763)
Target Microorganisms: Lactic acid bacteria, yeast and mold

Application

Wallerstein Nutrient Broth is used in the examination of materials encountered in brewing and for industrial fermentations containing mixed flora of yeast and bacteria. It is used in the beverage industry to detect bacteria, yeast, and mold in wines, beer and other fermentation products.

Features and Benefits

  • Ready-to-use Liquid ampoule media solve numerous problems in microbiology laboratories where time, equipment, and trained personnel are often in short supply.
  • They are available in convenient, single-test 2 mL plastic ampoules, eliminating the manipulation of glass ampoules and avoiding the waste and spoilage associated with bulk packaged media.
Incubation Temperature: 35 °C ± 2 for bacteria; 30 °C ± 2 for yeasts and molds
Incubation Time: 48–72 hrs

General description

Wallerstein Nutrient Broth is recommended for the isolation and cultivation of yeasts, molds and bacteria that grow in a low pH environment mainly in the beverage industry. Yeast extract in the medium is a source of trace elements, vitamins and amino acids. Peptone provides nitrogen, amino acids and carbon. Dextrose is a source of carbohydrates. Monopotassium phosphate buffers the media. Potassium chloride, calcium chloride, and ferric chloride are essential ions to help to maintain osmotic balance. Magnesium sulfate and manganese sulfate are sources of divalent cations. Bromocresol green is the pH indicator. The WL Nutrient medium plate incubated under aerobic conditions enables the growth of mainly yeast colonies, acetic acid bacteria, flavobacterium, proteus and thermophilic bacteria and under anaerobic condition enables the growth of lactic acid bacteria and Pediococcus. Bacterial colonies appear as non-pigmented to white, with a smooth or rough texture. Yeast appears as large creamy to white colonies.

Legal Information

MICROFIL is a registered trademark of Merck KGaA, Darmstadt, Germany
MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

动植物来源培养基
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Media for Isolation-Identification-Cultivation-Maintenance of Medical Bacteria
MacFaddin JF
Williams and Wilkins, 12, 353-353 (1985)
Paper read at American Society of Brewing Chemists Meeting
Green SR and Gray PP
Wallerstein Laboratories Communications, 12, 353-353 (1950)

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