Merck
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安全信息

MHA000P2N

Millipore

Wallerstein Nutrient Broth

ready-to-use, ampule of 2 mL, suitable for microbiology, for molds, for bacteria, for yeasts

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别名:
WL Nutrient broth, WLNB, Wallerstein Laboratory Nutrient Broth
eCl@ss:
32160801
NACRES:
NA.74

形式

liquid

质量水平

100

保质期

12 mo.

特点

ready-to-use

包装

ampule of 2 mL
pack of 50 tests

颜色

slightly opalescent green-blue

输入

sample type bottled water
sample type beer
sample type sport drink
sample type cider
sample type food(s)
sample type beverage(s)
sample type wine
sample type cosmetics
sample type soft drink
sample type beverage(s)

pH值（酸碱度）

5.5±0.2 (25.0 °C)

应用

environmental
food and beverages
industrial qc
microbiology

相容性

for use with MF-method
for use with Microfil®
for use with MicropreSure®
for use with Milliflex®

运输

wet ice

储存温度

2-8°C

适用性

bacteria
molds
yeasts

相关类别

一般描述

Wallerstein Nutrient Broth is recommended for the isolation and cultivation of yeasts, molds and bacteria that grow in a low pH environment mainly in the beverage industry. Yeast extract in the medium is a source of trace elements, vitamins and amino acids. Peptone provides nitrogen, amino acids and carbon. Dextrose is a source of carbohydrates. Monopotassium phosphate buffers the media. Potassium chloride, calcium chloride, and ferric chloride are essential ions to help to maintain osmotic balance. Magnesium sulfate and manganese sulfate are sources of divalent cations. Bromocresol green is the pH indicator. The WL Nutrient medium plate incubated under aerobic conditions enables the growth of mainly yeast colonies, acetic acid bacteria, flavobacterium, proteus and thermophilic bacteria and under anaerobic condition enables the growth of lactic acid bacteria and Pediococcus. Bacterial colonies appear as non-pigmented to white, with a smooth or rough texture. Yeast appears as large creamy to white colonies.

应用

Wallerstein Nutrient Broth is used in the examination of materials encountered in brewing and for industrial fermentations containing mixed flora of yeast and bacteria. It is used in the beverage industry to detect bacteria, yeast, and mold in wines, beer and other fermentation products.

特点和优势

  • Ready-to-use Liquid ampoule media solve numerous problems in microbiology laboratories where time, equipment, and trained personnel are often in short supply.
  • They are available in convenient, single-test 2 mL plastic ampoules, eliminating the manipulation of glass ampoules and avoiding the waste and spoilage associated with bulk packaged media.

产品规格

Incubation Temperature: 35 °C ± 2 for bacteria; 30 °C ± 2 for yeasts and molds
Incubation Time: 48–72 hrs

分析说明

QC organisms
发酵乳杆菌 (ATCC 9338)

酿酒酵母 (ATCC 9763)
细菌菌落可显示为非色素至白色，具有平滑或粗燥纹理。 酵母显示为更大的乳白色、白色菌落。
Target Microorganisms: Lactic acid bacteria, yeast and mold

法律信息

MICROFIL is a registered trademark of Merck KGaA, Darmstadt, Germany
MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

分析证书(COA)

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Paper read at American Society of Brewing Chemists Meeting
Green SR and Gray PP
Wallerstein Laboratories Communications, 12, 353-353 (1950)
Media for Isolation-Identification-Cultivation-Maintenance of Medical Bacteria
MacFaddin JF
Williams and Wilkins, 12, 353-353 (1985)

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