MHA000P2N
Wallerstein Nutrient Broth
ampoule capacity 2 mL, ampoule filling volume 2 mL, ready-to-use, pack of 50 tests, for bacteria, for yeasts, for molds
别名:
WL Nutrient broth, WLNB, Wallerstein Laboratory Nutrient Broth
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关于此项目
UNSPSC Code:
41106225
NACRES:
NA.74
eCl@ss:
32160801
Feature:
ready-to-use
Ph:
5.5±0.2 (25.0 °C)
sterility
sterile
form
liquid
shelf life
12 mo.
feature
ready-to-use
packaging
pack of 50 tests
ampoule capacity
2 mL
ampoule filling volume
2 mL
color
slightly opalescent green-blue
pH
5.5±0.2 (25.0 °C)
Quality Level
application(s)
environmental
food and beverages
industrial qc
microbiology
compatibility
for use with MF-method, for use with Microfil®, for use with MicropreSure®, for use with Milliflex®
shipped in
wet ice
storage temp.
2-8°C
suitability
bacteria, molds, yeasts
input
sample type bottled water
sample type beer
sample type sport drink
sample type cider
sample type food(s)
sample type wine
sample type cosmetics
sample type soft drink
General description
Wallerstein Nutrient Broth is recommended for the isolation and cultivation of yeasts, molds and bacteria that grow in a low pH environment mainly in the beverage industry. Yeast extract in the medium is a source of trace elements, vitamins and amino acids. Peptone provides nitrogen, amino acids and carbon. Dextrose is a source of carbohydrates. Monopotassium phosphate buffers the media. Potassium chloride, calcium chloride, and ferric chloride are essential ions to help to maintain osmotic balance. Magnesium sulfate and manganese sulfate are sources of divalent cations. Bromocresol green is the pH indicator. The WL Nutrient medium plate incubated under aerobic conditions enables the growth of mainly yeast colonies, acetic acid bacteria, flavobacterium, proteus and thermophilic bacteria and under anaerobic condition enables the growth of lactic acid bacteria and Pediococcus. Bacterial colonies appear as non-pigmented to white, with a smooth or rough texture. Yeast appears as large creamy to white colonies.
Application
Wallerstein Nutrient Broth is used in the examination of materials encountered in brewing and for industrial fermentations containing mixed flora of yeast and bacteria. It is used in the beverage industry to detect bacteria, yeast, and mold in wines, beer and other fermentation products.
Features and Benefits
- Ready-to-use Liquid ampoule media solve numerous problems in microbiology laboratories where time, equipment, and trained personnel are often in short supply.
- They are available in convenient, single-test 2 mL plastic ampoules, eliminating the manipulation of glass ampoules and avoiding the waste and spoilage associated with bulk packaged media.
Incubation Temperature: 35 °C ± 2 for bacteria; 30 °C ± 2 for yeasts and molds
Incubation Time: 48–72 hrs
Analysis Note
Bacterial colonies can appear non-pigmented to white, with a smooth or rough texture. Yeast appear as creamy, white larger colonies
QC organisms
L. fermentum (ATCC 9338)
S. cerevisiae (ATCC 9763)
L. fermentum (ATCC 9338)
S. cerevisiae (ATCC 9763)
Target Microorganisms: Lactic acid bacteria, yeast and mold
Legal Information
MICROFIL is a registered trademark of Merck KGaA, Darmstadt, Germany
MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
动植物来源培养基
此项目有
Media for Isolation-Identification-Cultivation-Maintenance of Medical Bacteria
MacFaddin JF
Williams and Wilkins, 12, 353-353 (1985)
Paper read at American Society of Brewing Chemists Meeting
Green SR and Gray PP
Wallerstein Laboratories Communications, 12, 353-353 (1950)
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