NA03
抗-PCNA（Ab-1）小鼠单克隆抗体（PC10）
liquid, clone PC10, Calbiochem®
别名:
抗-增殖细胞核抗原
登录 查看组织和合同定价。
选择尺寸
关于此项目
NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
PC10, monoclonal
Species reactivity:
rat, yeast, mouse, human, zebrafish
Application:
Citations:
72
产品名称
抗-PCNA（Ab-1）小鼠单克隆抗体（PC10）, liquid, clone PC10, Calbiochem®
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
PC10, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
rat, yeast, mouse, human, zebrafish
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(Immunoblotting (2.5 µg/mL)
Immunofluorescence (1-5 µg/mL)
Immunoprecipitation (not recommended with unconjugated antibody)
Paraffin Sections (2.5 µg/mL, no pre-treatment required))
isotype
IgG2a
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PCNA(5111)
zebrafish ... Pcna(30678)
Analysis Note
阳性对照
HeLa细胞、HL-60细胞、扁桃体组织或正常结肠组织
HeLa细胞、HL-60细胞、扁桃体组织或正常结肠组织
Application
免疫印迹（2.5 µg/mL）
免疫荧光（1-5 µg/mL）
免疫沉淀（不建议使用未结合的抗体，请参见注释）
石蜡切片（2.5 µg/mL，无需预处理，请参见注释）
免疫荧光（1-5 µg/mL）
免疫沉淀（不建议使用未结合的抗体，请参见注释）
石蜡切片（2.5 µg/mL，无需预处理，请参见注释）
Disclaimer
毒性：标准处理（A）
General description
识别HeLa细胞以及扁桃体或正常结肠组织中的~37 kDa PCNA蛋白。
该抗PCNA（Ab-1）小鼠mAb（PC10）经验证可用于免疫印迹、免疫荧光、免疫沉淀石蜡切片以检测PCNA（Ab-1）。
通过使用指定的免疫原免疫BALB/c小鼠并将脾细胞与Sp2/0-Ag14小鼠骨髓瘤细胞融合而产生的纯化的小鼠单克隆抗体。识别~37 kDa PCNA蛋白。
Immunogen
表位：氨基酸112-121内
重组PCNA
Other Notes
Waseem, N.H. and Lane, D.P.1990.J. Cell.Sci.96, 121.
Suzuka, I., et al. 1989.Proc.Natl.Acad.Sci. USA86 3189.
Bravo, R., et al. 1987.Nature (London)326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987.J. Cell.Biol.105, 1549.
Miyachi, K., et al. 1987.J. Immunol.121, 2228.
Suzuka, I., et al. 1989.Proc.Natl.Acad.Sci. USA86 3189.
Bravo, R., et al. 1987.Nature (London)326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987.J. Cell.Biol.105, 1549.
Miyachi, K., et al. 1987.J. Immunol.121, 2228.
染色显示出强烈的核模式。不建议用于免疫沉淀，因为在某些样品中会沉淀出约37 kDa的蛋白质，而不是PCNA。通常，石蜡切片染色不需要预处理，但是在某些样品中，可以通过在37°C下用4 N HCl预处理10分钟，然后在水中洗涤3次来增强染色。在单个系统中，应对抗体进行滴定以获得最佳结果。
Packaging
批次特定浓度请参见小瓶标签。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
未找到合适的产品？
试试我们的产品选型工具.
存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Hailong Wang et al.
Journal of the American Heart Association, 8(14), e011994-e011994 (2019-07-13)
Background Exposure to chronic psychosocial stress is a risk factor for atherosclerosis-based cardiovascular disease. We previously demonstrated the increased expressions of cathepsin S (CatS) in atherosclerotic lesions. Whether CatS participates directly in stress-related neointimal hyperplasia has been unknown. Methods and
Mayu Inokuchi et al.
The Journal of experimental biology, 220(Pt 24), 4720-4732 (2017-11-01)
Spatiotemporal changes in branchial ionocyte distribution were investigated following transfer from seawater (SW) to freshwater (FW) in Japanese seabass. The mRNA expression levels of cystic fibrosis transmembrane conductance regulator (CFTR) and Na+/K+/2Cl- cotransporter 1a (NKCC1a) in the gills rapidly decreased
B K Law et al.
The Journal of biological chemistry, 275(49), 38261-38267 (2000-09-20)
Salicylate and its pro-drug form aspirin are widely used medicinally for their analgesic and anti-inflammatory properties, and more recently for their ability to protect against colon cancer and cardiovascular disease. Despite the wide use of salicylate, the mechanisms underlying its
Alfeu Zanotto-Filho et al.
Cancer letters, 425, 101-115 (2018-04-03)
Molecular targeted compounds are emerging as a strategy to improve classical chemotherapy. Herein, we describe that using low dose of the multikinase inhibitor sorafenib improves cyclophosphamide antitumor activity by inhibiting angiogenesis, metastasis and promoting tumor healing in MDA-MB231 xenografts and
K Henke et al.
International journal of radiation oncology, biology, physics, 36(2), 385-391 (1996-09-01)
Stereotactic radiosurgery (RS) is being used increasingly for the treatment of small benign and malignant lesions, particularly in the brain. However, to fully realize the potential of this technique, more experimental data are needed. In this report we describe an
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持