产品名称
5-甲基胞嘧啶小鼠单克隆抗体（162 33 D3）, liquid, clone 162 33 D3, Calbiochem®
biological source
mouse
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
162 33 D3, monoclonal
form
liquid
does not contain
preservative
species reactivity (predicted by homology)
all
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(ELISA (0.1 µg/mL)
Flow Cytometry (1 µg/mL)
Frozen Sections (5-10 µg/mL)
Immunoblotting (5 µg/mL)
Immunofluorescence (1 µg/mL)
Paraffin Sections (5-10 µg/mL)
Radioimmunoassay (0.1 µg/mL)
Southwestern Blot (5 µg/mL))
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Analysis Note
阳性对照
NIH3T3细胞
NIH3T3细胞
Application
ELISA（0.1 g/mL）
流式细胞术（1 g/mL）
冷冻切片（5-10 g/mL）
免疫印迹法（5 g/mL）
免疫荧光（1 g/mL）
石蜡切片（5-10 g/mL）
放射免疫分析（0.1 g/mL）
DNA-蛋白质印迹法（5 g/mL）
Disclaimer
毒性：标准处理（A）
General description
免疫亲和纯化的小鼠单克隆抗体。识别5-甲基胞嘧啶。
经验证，该抗5-甲基胞嘧啶小鼠mAb（162 33 D3）可用于ELISA、FC、冷冻切片、IF、石蜡切片、放射免疫分析、DNA-蛋白质印迹法检测5-甲基胞嘧啶。
识别NIH3T3细胞中甲基化DNA或RNA中的5-甲基胞嘧啶。
特异性
•亲和纯化的小鼠单克隆抗体 - 可检测多种物种的甲基化DNA。几种应用
• 流式细胞术
•冷冻切片
• 免疫印迹
•免疫荧光
•石蜡切片
可靠
•产生可重复的结果。
•克隆162 33 D3已在50多个出版物中使用。
特异性
•亲和纯化的小鼠单克隆抗体 - 可检测多种物种的甲基化DNA。几种应用
• 流式细胞术
•冷冻切片
• 免疫印迹
•免疫荧光
•石蜡切片
可靠
•产生可重复的结果。
•克隆162 33 D3已在50多个出版物中使用。
Immunogen
5-甲基胞嘧啶结合卵清蛋白
Other Notes
Ehrlich, M. 2002.Oncogene21, 5400.(Review)
Widschwendter, M. and Jones, P.A.2002.Oncogene21, 5462.(Review)
Barton, S.C., et al. 2001.Hum.Mol.Genet.10, 2983.
Piyathilake, C.J., et al. 2001.Hum.Pathol.32, 856.
Taddei, A., et al. 2001.Nat. Cell Biol.3, 114.
Piyathilake, C.J., et al. 2000.Biotech.Histochem.75, 251.
De Capoa, A., et al. 1999.FASEB J.13, 89.
Widschwendter, M. and Jones, P.A.2002.Oncogene21, 5462.(Review)
Barton, S.C., et al. 2001.Hum.Mol.Genet.10, 2983.
Piyathilake, C.J., et al. 2001.Hum.Pathol.32, 856.
Taddei, A., et al. 2001.Nat. Cell Biol.3, 114.
Piyathilake, C.J., et al. 2000.Biotech.Histochem.75, 251.
De Capoa, A., et al. 1999.FASEB J.13, 89.
克隆162 33 D3可用于定量和定性检测各种样品和应用中的甲基化DNA或RNA。它已在40多个出版物中使用。应滴定抗体，以在单个系统中获得最佳结果。
Packaging
批次特定浓度请参见小瓶标签。
Physical form
在PBS（pH 7.4）中。
Preparation Note
初次解冻后，等分并冷冻（-20°C）。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
未找到合适的产品？
试试我们的产品选型工具.
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Dayanidhi Kumar et al.
PloS one, 8(7), e69927-e69927 (2013-08-08)
Cytogenetic studies have demonstrated that low levels of chronic radiation exposure can potentially increase the frequency of chromosomal aberrations and aneuploidy in somatic cells. Epidemiological studies have shown that health workers occupationally exposed to ionizing radiation bear an increased risk
Bo Xia et al.
International journal of toxicology, 35(3), 336-343 (2016-03-11)
Benzo[a]pyrene (B[a]P) exposure has been associated with the alteration in epigenetic marks that are involved in cancer development. Biotinidase (BTD) and holocarboxylase synthetase (HCS) are 2 major enzymes involved in maintaining the homeostasis of biotinylation, and the deregulation of this
Jun-Xue Jin et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 41(3), 1255-1266 (2017-03-08)
Hypoacetylation caused by aberrant epigenetic nuclear reprogramming results in low efficiency of mammalian somatic cell nuclear transfer (SCNT). Many epigenetic remodeling drugs have been used in attempts to improve in vitro development of porcine SCNT embryos. In this study, we
Ikuo Matsuda et al.
Journal of clinical and experimental hematopathology : JCEH, 54(1), 67-73 (2014-06-20)
Lymphomas are malignant neoplasms composed of lymphoid cells at various developmental stages and lineages. Recent advances in comprehensive genomic analyses in acute myeloid leukemia have revealed prevalent mutations in regulators of epigenetic phenomena including global DNA methylation status. The examples
Gu Zhou et al.
BioTechniques, 62(4), 157-164 (2017-04-14)
Here, we present a DNA restriction enzyme-based, fluorescent cytosine extension assay (CEA) to improve normalization and technical variation among sample-to-sample measurements. The assay includes end-labeling of parallel methylation-sensitive and methylation-insensitive DNA restriction enzyme digests along with co-purification and subsequent co-measurement
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持