产品名称
降钙素基因相关肽（CGRP）兔多克隆抗体, lyophilized, Calbiochem®
biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized
contains
≤0.1% sodium azide as preservative
species reactivity
rat, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(Free Floating Sections
Frozen Sections (1:2000-1:4000, ABC detection)
Immunofluorescence (1:100-1:200,
Paraffin Sections (s; heat pre-treatment required; ))
isotype
IgG
shipped in
ambient
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
mouse ... Calca(12310)
Analysis Note
阳性对照
大鼠杏仁核或脊髓
大鼠杏仁核或脊髓
Application
自由浮动切片（参见应用参考文献）
冷冻切片（1:2000-1:4000，ABC检测）
免疫荧光（1:100-1:200，请参阅应用参考文献）
石蜡切片（请参阅应用参考文献；需要预先热处理；参见注释）
Disclaimer
毒性：标准处理（A）
General description
兔多克隆抗CGRP可识别大鼠杏仁核和脊髓中的α-CGRP。它已被验证可用于免疫荧光，&可与冷冻、石蜡和自由浮动切片一起使用。
兔多克隆抗体以未稀释的血清形式提供。可识别α-CGRP蛋白。
可识别大鼠杏仁核和脊髓中的α-降钙素基因相关肽。
Immunogen
大鼠α-降钙素基因相关肽（α-CGRP）
Other Notes
在大鼠杏仁核和脊髓中检查了抗体特异性。用10-5M大鼠α-CGRP预处理抗体可完全消除染色，用大鼠β-CGRP预吸收可部分消除染色。降钙素的预吸收不会导致免疫染色的损失。对于石蜡切片染色，将切片在40 mM枸橼酸钠缓冲液（pH 8.5）中加热至80°C。在单个系统中，应对抗体进行滴定以获得最佳结果。
Preparation Note
用100 µl无菌蒸馏H₂O复溶冻干抗体。注意复溶小瓶的全部内容物；在运输和处理过程中，冻干颗粒的部分可能已脱落，可能不在小瓶底部。溶解后，等分并冷冻保存（-20°C）。储备溶液在-20°C下可稳定保存至多6个月。用无菌PBS或Tris缓冲液稀释，稀释度不高于1:10。避免溶液反复冻融。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Although there are many candidates as molecular mechanotransducers, so far there has been no evidence for molecular specialization of visceral afferents. Here, we show that colonic afferents express a specific molecular transducer that underlies their specialized mechanosensory function: the transient
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The NLRP3 inflammasome senses diabetic metabolites and initiates inflammation implicated in diabetic complications and neurodegeneration. No studies have investigated NLRP3 in diabetic bladder dysfunction (DBD), despite a high clinical prevalence. In vitro, we found that numerous diabetic metabolites activate NLRP3
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