biological source
mouse
Quality Level
antibody form
unpurified
antibody product type
primary antibodies
clone
FK2, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity (predicted by homology)
all
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA
Immunoblotting (1:1000)
Immunoprecipitation (s and comments)
Immunofluorescence )
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
可识别K29-、K48-和K63-连接的多泛素化和单泛素化蛋白，但不能识别Raji细胞中的游离泛素。
抗泛素小鼠mAb（FK2）经过验证可用于ELISA、免疫印迹、免疫沉淀和免疫荧光法，以检测泛素。
通过使用指定的免疫原免疫BALB/c小鼠并将脾细胞与骨髓瘤细胞融合而产生的小鼠单克隆抗体。可识别K29-、K48-和K63-连接的多泛素化和单泛素化蛋白。
Immunogen
人
未纯化的多聚泛素化溶菌酶
Application
ELISA（参见应用参考文献）
免疫印迹法（1:1000）
免疫沉淀法（参见应用参考文献和备注）
免疫荧光法（见应用参考文献）
Packaging
请参考特定浓度批号的标签。
Physical form
溶于PBS中。
Preparation Note
Raji细胞
初次融化后，等分并冷冻（-20°C）。稀释抗体可在4°C下储存最长1个月。
Other Notes
Matsumoto, M., et al. 2005.Proteomics 5, 4545.
Boname, J.N., et al. 2001.Immunity 15, 627.
Campbell, V., et al. 2001.Neuron 32, 1013.
Fujimuro, M., et al. 2003.FEBS Letts.349, 173.
Boname, J.N., et al. 2001.Immunity 15, 627.
Campbell, V., et al. 2001.Neuron 32, 1013.
Fujimuro, M., et al. 2003.FEBS Letts.349, 173.
不能识别游离泛素。对于免疫沉淀，一旦抗体被固定，它就显示出对游离泛素以及多泛素化和单泛素化蛋白的亲和力。在单个系统中，应对抗体进行滴定以获得最佳结果。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性：标准处理（A）
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| ST1200-100UG | 04055977224078 |