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TMS-008

Sigma-Aldrich

Alizarin-Red Staining Solution

Alizarin red is a commonly used stain to identify calcium containing osteocytes in differentiated culture of both human and rodent mesenchymal stem cells (MSCs).

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别名:
Alizarin Red S, Alizarin Red S Stain, Alizarin Red Stain
NACRES:
NA.75

质量水平

浓度

40 mM in distilled water

技术

cell based assay: suitable
cell culture | stem cell: suitable
cell differentiation: suitable

一般描述

Alizarin Red, an anthraquinone derivative, may be used to identify calcium in tissue sections and cultured cells in vitro. The reaction is not strictly specific for calcium, since magnesium, manganese, barium, strontium, and iron may interfere, but these elements usually do not occur in sufficient concentration to interfere with the staining. Calcium forms an Alizarin Red S-calcium complex in a chelation process, and the end product is a bright red stain. Alizarin red is a commonly used stain to identify calcium containing osteocyte in differentiated culture of both human and rodent mesenchymal stem cells.

应用

Research Category
Stem Cell Research
Research Sub Category
Osteobiology

Arthritis

质量

Solution is red with no particulates present. Solution is suitable for endpoint cell culture assays.

外形

Product is presented as a 40mM solution at pH 4.1 +/- 0.1.

储存及稳定性

Store at 2-8ºC for up to 4 months from date of receipt. If Alizarin-red solution forms a precipitate, remove particulates by passage through a 0.22 or 0.45-micron filter.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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商品

Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.

新型无血清、无异种人间充质干细胞(MSC)骨细胞分化培养基的开发。

PLTMax® Human Platelet Lysate (hPL) is a superior serum-free and xeno-free media supplement alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) cultures.

Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.

实验方案

有关间充质的信息,特别是间充质干细胞培养实验方案用于间充质干细胞(MSC)分离、扩增和分化的分步细胞培养方案。

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

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