Immobilon^® 封闭液 - PO（磷蛋白封闭液）

The possible causes and potential remedies for challenges encountered as a result of blocking, washing, antibody incubation, and detection/exposure of Western blots

蛋白磷酸化是一种可逆的翻译后加工，在细胞内信号传递中有非常重要的功能。采用Western Blotting技术检测磷酸化蛋白是探讨信号传导中上游调控、下游功能调整、信息交流及反馈机制等的基本手段。我们提供专用的优质试剂和研究方法，帮助你获得准确、灵敏的磷酸化检测数据。

我们明白。西部片的污点可能会让人头疼，我们当然也有过这样的经历。因此，请与我们分享您的丑陋污点，并获得一份惊喜！虽然我们不能告诉你是什么--那会破坏惊喜的气氛--但它会激发实验室的创造力。

We get it. Westerns can be a pain in the blot, and we’ve certainly made our share. So, share with us your ugly blots and receive a surprise! And while we can’t tell you what it is – that would spoil the surprise - except that it will spark creativity in the lab.

In Western blotting, blocking of unbound membrane sites is necessary to prevent non-specific binding of the antibodies which otherwise would lead to a high background on the blot. The traditional milk blocker can work well; however, inadvertent variations in its composition can lead to irreproducible results. Such variations include the milk’s concentration, fat content, solubility, detergent quality, and numerous other factors. Bløk reagents are a family of protein-free, noisecancelling reagents that reduce background for consistent, quality results. They are available in three room temperature-stable, ready-to-use formulations for Chemiluminescence and chromogenic detection, Fluorescence detection, and Phosphoprotein immunodetection. The protein-free nature of Bløk reagents allows for membranes to be stained with chromogenic reagents, such as Ponceau S or Coomassie™ blue, after blocking or immunodetection. Bløk reagents have been tested and validated for Western blot, dot blot, and ELISA applications.