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Merck
CN

WBAVDP001

Millipore

Immobilon® 封闭液 - PO(磷蛋白封闭液)

Western blotting, dot/slot blotting, mass spectrometry, phosphorolation assay

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关于此项目

UNSPSC代码:
41116010
eCl@ss:
42029053
NACRES:
NB.22
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质量水平

包装

pkg of 500 mL

制造商/商品名称

Immobilon®

技术

western blot: suitable

膜面积

1000 cm2 , membrane coverage

检测方法

chemiluminescent (phosphoprotein)
fluorometric (phosphoprotein)

运输

ambient

储存温度

15-25°C

制备说明

在20–25°C条件下可稳定保存1年

分析说明

为了获得最佳结果,使用Immobilon-P膜进行化学发光(鲁米诺)或目视检测(例如TMB),并使用Immobilon-FL膜进行荧光检测。

法律信息

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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Johnathan M Borland et al.
International journal of molecular sciences, 24(2) (2023-01-22)
Like many social behaviors, aggression can be rewarding, leading to behavioral plasticity. One outcome of reward-induced aggression is the long-term increase in the speed in which future aggression-based encounters is initiated. This form of aggression impacts dendritic structure and excitatory
H N Gao et al.
Journal of dairy science, 100(9), 7696-7709 (2017-06-26)
The ratio of different AA in the diets of cows is vital to improve milk protein yield. β-Casein is one of the important milk proteins with high nutritive value. However, the suitable ratio of essential amino acids (EAA) for the
Vanesa D Ramseyer et al.
American journal of physiology. Renal physiology, 308(2), F149-F156 (2014-11-08)
Thick ascending limbs reabsorb 30% of the filtered NaCl load. Nitric oxide (NO) produced by NO synthase 3 (NOS3) inhibits NaCl transport by this segment. In contrast, chronic angiotensin II (ANG II) infusion increases net thick ascending limb transport. NOS3
Riasat Zaman et al.
The Journal of cell biology, 220(6) (2021-04-10)
Activated ezrin-radixin-moesin (ERM) proteins link the plasma membrane to the actin cytoskeleton to generate apical structures, including microvilli. Among many kinases implicated in ERM activation are the homologues LOK and SLK. CRISPR/Cas9 was used to knock out all ERM proteins
I-Ting Chen et al.
EMBO reports, 22(12), e52254-e52254 (2021-10-12)
Promyelocytic leukemia protein (PML) is a tumor suppressor possessing multiple modes of action, including induction of apoptosis. We unexpectedly find that PML promotes necroptosis in addition to apoptosis, with Pml-/- macrophages being more resistant to TNF-mediated necroptosis than wild-type counterparts

商品

The possible causes and potential remedies for challenges encountered as a result of blocking, washing, antibody incubation, and detection/exposure of Western blots

蛋白磷酸化是一种可逆的翻译后加工,在细胞内信号传递中有非常重要的功能。采用Western Blotting技术检测磷酸化蛋白是探讨信号传导中上游调控、下游功能调整、信息交流及反馈机制等的基本手段。我们提供专用的优质试剂和研究方法,帮助你获得准确、灵敏的磷酸化检测数据。

相关内容

We get it. Westerns can be a pain in the blot, and we’ve certainly made our share. So, share with us your ugly blots and receive a surprise! And while we can’t tell you what it is – that would spoil the surprise - except that it will spark creativity in the lab.

我们明白。西部片的污点可能会让人头疼,我们当然也有过这样的经历。因此,请与我们分享您的丑陋污点,并获得一份惊喜!虽然我们不能告诉你是什么--那会破坏惊喜的气氛--但它会激发实验室的创造力。

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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