biological source
Staphylococcus aureus
form
lyophilized
specific activity
~15,000 U/mg
packaging
pkg of 15,000 U
manufacturer/tradename
Roche
optimum pH
7.5
shipped in
wet ice
Quality Level
Application
- 从体外翻译系统中去除内源性RNA
- RNA测序实验
核酸酶S7已用于核糖体分析的核糖核酸酶选择过程研究。
Biochem/physiol Actions
核酸酶S7是一种核酸内切酶，主要切割单链底物。它还切割双链DNA或RNA。它切割RNA和DNA的5′磷酸二酯键，产生3′-单核苷酸和寡核苷酸。酶的活性取决于Ca2+ ，添加EDTA可使酶完全灭活。相比于DNA上的GC，核酸酶S7切割对富含AT的区域高度特异。
Preparation Note
未开封试剂储存于2-8 °C；1 mg/mL的稀释液则储存于-15 to -25 °C。
Other Notes
一单位酶释放的足够量的酸溶性寡核苷酸可使A260增加1.0。
仅用于生命科学研究。不可用于诊断。
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
常规特殊物品
此项目有
Organ-specific translation elongation rates measured by in vivo ribosome profiling.
Gerashchenko M V, et al.
bioRxiv, 279257 (2018)
High sequence specificity of micrococcal nuclease.
Dingwall C, et al.
Nucleic Acids Research, 9(12), 2659-2674 (1981)
Sequence specific cleavage of African green monkey ?-satellite DNA by micrococcal nuclease.
Horz W, et al.
Nucleic Acids Research, 11(13), 4275-4285 (1983)
Maxim V Gerashchenko et al.
Nucleic acids research, 49(2), e9-e9 (2020-12-03)
There has been a surge of interest towards targeting protein synthesis to treat diseases and extend lifespan. Despite the progress, few options are available to assess translation in live animals, as their complexity limits the repertoire of experimental tools to
Ribonuclease selection for ribosome profiling.
Gerashchenko M V and Gladyshev V N
Nucleic Acids Research, 45(2), e6-e6 (2016)
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