biological source
bovine pancreas
assay
≥90%
form
lyophilized (salt-free)
specific activity
≥70 units/mg protein (at 25 °C, with ATEE as a substrate)
mol wt
Mr 25 kDa
packaging
pkg of 4 × 25 μg
manufacturer/tradename
Roche
technique(s)
protein sequencing: suitable
optimum pH
7.0-9.0
shipped in
wet ice
storage temp.
2-8°C
General description
测序级胰凝乳蛋白酶是从牛胰腺中分离得到的纯化的特异性蛋白酶。
Application
单独使用测序级胰凝乳蛋白酶或者与其他蛋白酶组合使用，对蛋白质进行水解。它适用于肽谱分析、指纹图谱分析和序列分析。
Biochem/physiol Actions
丝氨酸内肽酶能够特异性水解Tyr、Phe和Trp的C末端肽键。Leu、Met、Ala、Asp和Glu的水解速率较低。水解速率还与目标氨基酸的酰胺和酯相关。测序级胰凝乳蛋白酶的特异性是以蜂毒肽作为底物进行测试的。
Preparation Note
工作浓度：建议酶用量为蛋白质重量的1/200到1/20。
储存条件（工作溶液）：2至8 °C
溶于1 mM HCl的溶液可在2至8°C保存最长1周。
抑制剂：抑肽酶，DFP，PMSF，吩噻嗪-N-碳酰氯，TPCK，ZPCK，α2-巨球蛋白，α1-抗胰蛋白酶，大豆胰蛋白酶抑制剂和胰凝乳蛋白酶抑制剂。APMSF对该酶无抑制作用。
储存条件（工作溶液）：2至8 °C
溶于1 mM HCl的溶液可在2至8°C保存最长1周。
抑制剂：抑肽酶，DFP，PMSF，吩噻嗪-N-碳酰氯，TPCK，ZPCK，α2-巨球蛋白，α1-抗胰蛋白酶，大豆胰蛋白酶抑制剂和胰凝乳蛋白酶抑制剂。APMSF对该酶无抑制作用。
将冻干的测序级胰凝乳蛋白酶溶解在1mM HCl中。将待测序蛋白质溶于消化缓冲液（100mM Tris-HCl，10mM CaCl2，pH7.8）中。
Analysis Note
纯度：反相HPLC分析显示，不含可能干扰特异性裂解或肽分离的杂质。
Other Notes
仅用于生命科学研究。不可用于诊断。
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
动植物源性产品
低风险生物材料
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