biological source
bovine pancreas
form
dry powder
specific activity
50 U/mg (+25°C, RNA as substrate)
mol wt
Mr = 13,700
packaging
pkg of 100 mg (10109169001), pkg of 25 mg (10109142001)
manufacturer/tradename
Roche
parameter
65 °C optimum reaction temp.
technique(s)
flow cytometry: suitable
optimum pH
7.0-7.5
storage temp.
2-8°C
General description
牛胰腺核糖核酸酶A是核糖核酸酶A蛋白质超家族的成员之一。它是最具有特征的蛋白质，是肾脏形状的基本蛋白质。该蛋白质由124个氨基酸组成。核糖核酸酶A的天然形式是同二聚体。
Application
- 用于分析目的的
- DNA提取（如果是用于分析，核糖核酸酶A需要煮沸破坏）
- 流式细胞术细胞周期分析和碘化丙啶(PI)染色
Biochem/physiol Actions
核糖核酸酶A催化RNA转磷酸化和降解。它是第一种被确认具有使DNA变性功能的蛋白质。该酶能够和单链（ss）RNA聚核苷酸链上多个结合位点结合，然后降解它。它和身上DNA以相似的方式发生作用，导致DNA解旋。通过串联获得的核糖核酸酶A二聚体对癌症细胞有毒性。
Preparation Note
核糖核酸酶A在10mM Tris-HCl,pH7.5,15mM NaCl中溶解，浓度为1到10mg/ml。加热到100℃，15分钟使得里面的脱氧核糖核酸酶失活，然后逐渐降温到室温，分装。Roche建议随后储存在-15到-25℃。
Other Notes
一单位酶活性导致A0比A1吸收率在实验条件下降低。A0 比A1对应总转化，A1是终吸收率。
仅用于生命科学研究。不可用于诊断。
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1
法规信息
低风险生物材料
动植物源性产品
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 10109142001 | 04061838667007 |
| 10109169001 | 04061837684449 |