02538
Raka-Ray 琼脂
NutriSelect® Plus, powder, selective for lactic acid bacteria, pack of 500 g, suitable for microbiology
别名:
乳酸菌选择性琼脂基础
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关于此项目
NACRES:
NA.85
UNSPSC Code:
41171609
Manufacturer/tradename:
NutriSelect® Plus
Form:
powder
Suitability:
Pediococcus spp., selective for lactic acid bacteria
Application(s):
agriculture
bioburden testing
food and beverages, microbiology
bioburden testing
food and beverages, microbiology
sterility
non-sterile
Quality Segment
form
powder
shelf life
limited shelf life, expiry date on the label
composition
N-acetyl glucosamine, 0.5 g/L , agar, 17.0 g/L , betaine hydrochloride, 2.0 g/L , casein enzymic hydrolysate, 20.0 g/L , diammonium hydrogen-citrate, 2.0 g/L , fructose, 5.0 g/L , glucose, 5.0 g/L , liver concentrate, 1.0 g/L , magnesium sulphate, 2.0 g/L , maltose, 10.0 g/L , manganese sulfate, 0.66 g/L , monopotassium phosphate, 2.0 g/L , potassium aspartate, 2.5 g/L , potassium glutamate, 2.5 g/L , yeast extract, 5.0 g/L
packaging
pack of 500 g
manufacturer/tradename
NutriSelect® Plus
final pH
5.4±0.2 (25 °C)
application(s)
agriculture
bioburden testing
food and beverages, microbiology
storage temp.
2-8°C
suitability
Pediococcus spp., selective for lactic acid bacteria
General description
Raka-Ray Agar was formulated by Saha, Sondag and Middlekauff to monitor the brewing process and analyze it for a wide range of bacteria. The media is found to be superior to several other media for the cultivation of Lactobacilli and Pediococci. It also suppresses the growth of non-lactic acid facultative bacteria that are often associated with lactic beer spoilage.
Application
Raka-Ray Agar is recommended for selective isolation of lactic acid bacteria encountered in beer and the brewing process.
Preparation Note
Suspend 38.5 g in 500 ml of distilled water. Bring to the boil and dissolve the medium completely. Distribute into tubes or bottles and sterilize by autoclaving at 121°C for 15 minutes. Cool to 50-55°C and add contents of 1vial of lactic acid bacteria selective supplement (Cat. No. 14121). Add 3.5 ml of Cycloheximide solution (Cat. No. 18079). Mix well and pour into sterile petri dishes or dispense as desired.
Analysis Note
Casein enzymic hydrolysate, liver concentrate, yeast extract provide carbon, nitrogen, amino acids, minerals, vitamins, trace elements and other essential nutrients for growth. Potassium aspartate and potassium glutamate are additional sources of amino acids. Maltose is to detect lactobacilli, which cannot utilize glucose as a carbon source and fructose is the carbon source of Lactobacillus fructivorans. Glucose is needed as the fermentative energy source for the Pediococci. Sorbitan mono-oleate act as a stimulant for lactic acid bacteria in general. Also, N-acetyl glucosamine, liver concentrate yeast extract and betaine hydrochloride are used as growth stimulating agents. Diammonium hydrogen-citrate and Monopotassium phosphate are the buffering agents while Magnesium and Manganese are important trace elements for Lactobacillaceae. The addition of phenylethanol inhibits Gram-negative organisms, while amphotericin B and cycloheximide inhibits growth of yeasts and molds.
Other Notes
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
存储类别
11 - Combustible Solids
ppe
Eyeshields, Gloves, type N95 (US)