114375
溴甲酚紫
BioReagent, suitable for indicator, Dye content 90 %
别名:
5,5′-二溴-邻甲酚磺酰酞, 溴甲酚紫磺酸内酯
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关于此项目
经验公式（希尔记法）:
C21H16Br2O5S
化学文摘社编号:
分子量:
540.22
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
204-087-8
Beilstein/REAXYS Number:
359618
MDL number:
InChI key
ABIUHPWEYMSGSR-UHFFFAOYSA-N
InChI
1S/C21H16Br2O5S/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21/h3-10,24-25H,1-2H3
SMILES string
Cc1cc(cc(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C)c(O)c(Br)c4
product line
BioReagent
assay
≥89.0% (HPLC)
form
powder
composition
Dye content, 90%
technique(s)
titration: suitable
pH
5.2-6.8, yellow to purple
visual transition interval
5.2-6.8, yellow to blue
mp
240 °C (dec.) (lit.)
solubility
ethanol: 1 mg/mL
λmax
419 nm
ε (extinction coefficient)
≥22000 at 418-424 nm in methanol
suitability
suitable for indicator
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
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Application
溴甲酚紫已用于染色 酿酒酵母 。
血清-白蛋白的分光光度测定
Biochem/physiol Actions
溴甲酚紫是一种 pH 指示剂，可将颜色从黄色（低至 pH 5.2）变为紫色（高于 pH 6.8）。主要用作酵母细胞中的荧光染料。有助于细胞膜损伤细胞的死细胞计数。细胞膜正常的细胞不被染色，而受损的细胞则为蓝灰色重影。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
Yeast colonies synchronise their growth and development.
Palkova Z and Forstova J
Journal of Cell Science, 113, 1923-1928 (2000)
ATP leakage from yeast cells treated by extracellular glycolipids of Pseudozyma fusiformata.
Kulakovskaya TV, et al.
FEMS Yeast Research, 3, 401-404 (2003)
Fluorescent staining with bromocresol purple: a rapid method for determining yeast cell dead count developed as an assay of killer toxin activity.
Kurzweilova H and Sigler K
Yeast, 9, 1207-1211 (1993)
S M Al-Ghannam
Journal of pharmaceutical and biomedical analysis, 40(1), 151-156 (2005-08-23)
A simple, extraction-free spectrophotometric method is proposed for the analysis of some beta-blockers, namely atenolol, timolol and nadolol. The method is based on the interaction of the drugs in chloroform with 0.1% chloroformic solutions of acidic sulphophthalein dyes to form
Lydie Maresová et al.
BioTechniques, 43(5), 667-672 (2007-12-13)
Microplate readers have been useful assistants of researchers for several decades. This work is focused on the applications of a simple absorbance microplate reader in yeast physiology research, and its advantages and limitations in comparison with alternative methods are discussed.
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