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Merck
CN

114375

溴甲酚紫

BioReagent, suitable for indicator, Dye content 90 %

别名:

5,5′-二溴-邻甲酚磺酰酞, 溴甲酚紫磺酸内酯

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关于此项目

经验公式(希尔记法):
C21H16Br2O5S
化学文摘社编号:
分子量:
540.22
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
204-087-8
Beilstein/REAXYS Number:
359618
MDL number:
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InChI key

ABIUHPWEYMSGSR-UHFFFAOYSA-N

InChI

1S/C21H16Br2O5S/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21/h3-10,24-25H,1-2H3

SMILES string

Cc1cc(cc(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C)c(O)c(Br)c4

product line

BioReagent

assay

≥89.0% (HPLC)

form

powder

composition

Dye content, 90%

technique(s)

titration: suitable

pH

5.2-6.8, yellow to purple

visual transition interval

5.2-6.8, yellow to blue

mp

240 °C (dec.) (lit.)

solubility

ethanol: 1 mg/mL

λmax

419 nm

ε (extinction coefficient)

≥22000 at 418-424 nm in methanol

suitability

suitable for indicator

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

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Application

溴甲酚紫已用于染色 酿酒酵母
血清-白蛋白的分光光度测定

Biochem/physiol Actions

溴甲酚紫是一种 pH 指示剂,可将颜色从黄色(低至 pH 5.2)变为紫色(高于 pH 6.8)。主要用作酵母细胞中的荧光染料。有助于细胞膜损伤细胞的死细胞计数。细胞膜正常的细胞不被染色,而受损的细胞则为蓝灰色重影。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Fluorescent staining with bromocresol purple: a rapid method for determining yeast cell dead count developed as an assay of killer toxin activity.
Kurzweilova H and Sigler K
Yeast, 9, 1207-1211 (1993)
ATP leakage from yeast cells treated by extracellular glycolipids of Pseudozyma fusiformata.
Kulakovskaya TV, et al.
FEMS Yeast Research, 3, 401-404 (2003)
Yeast colonies synchronise their growth and development.
Palkova Z and Forstova J
Journal of Cell Science, 113, 1923-1928 (2000)
André Klauss et al.
Photosynthesis research, 102(2-3), 499-509 (2009-03-31)
Determination of thermodynamic parameters of water oxidation at the photosystem II (PSII) manganese complex is a major challenge. Photothermal beam deflection (PBD) spectroscopy determines enthalpy changes (ΔH) and apparent volume changes which are coupled with electron transfer in the S-state
M Arab Chamjangali et al.
Journal of hazardous materials, 166(2-3), 701-705 (2009-01-13)
A simple, selective, and sensitive kinetic spectrophotometric method is described for the determination of trace amounts of phenylhydrazine, which is based on its inhibition effect on the reaction between meta cresol purple (MCP) and periodate in the presence of bromide

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