sterility
non-sterile
form
powder
shelf life
limited shelf life, expiry date on the label
composition
agar, 15 g/L , casein enzymic hydrolysate, 10 g/L , sodium chloride, 10 g/L , yeast extract, 5 g/L
manufacturer/tradename
NutriSelect® Plus
technique(s)
microbiological culture: suitable
final pH
7.5±0.2 (25 °C)
application(s)
clinical testing
food and beverages
life science and biopharma, microbiology
suitability
nonselective for Escherichia coli, nonselective for bacteria (General Media), nonselective for coliforms
Quality Level
General description
LB-Agar, high salt is a growth media used in microbiology. It supports Escherichia coli growth to an optical density at 600 nm (OD600) of 7.
Application
LB-Agar, high salt may be used as a growth media in the determination of phylogenetic groups (A, B1, B2, and D) of Escherichia coli bacteria using triplex PCR technique.
Luria Media are used for the cultivation and maintenance of recombinant strains of E. coli for genetic and molecular studies.
Preparation Note
Suspend 40 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute. Sterilize at 121°C for 15 minutes. Dispense as desired.
Other Notes
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
Rapid and simple determination of theEscherichia coli phylogenetic group.
Clermont O, et al.
Applied and Environmental Microbiology, 66(10), 4555-4558 (2000)
Commercial Lysogeny Broth culture media and oxidative stress: a cautious tale.
Ezraty B, et al.
Free Radical Biology & Medicine, 74, 245-251 (2014)
Escherichia coli Physiology in Luria-Bertani Broth.
Sezonov G, et al.
Journal of Bacteriology, 189(23), 8746?8749-8746?8749 (2007)
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