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Merck
CN

60786

King 琼脂 B

NutriSelect® Basic, according to ISO 16266:2006, powder, nonselective and differential for Pseudomonas spp., pack of 500 g, suitable for microbiology

别名:

King′s Medium B, Pseudomonas Agar, F Agar, Fluorescein Agar, King′s B Medium, Pigment Production Agar B, Pseudomonas Agar (for Fluorescein)

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关于此项目

NACRES:
NA.74
UNSPSC Code:
41171606
Manufacturer/tradename:
NutriSelect® Basic
Agency:
according to ISO 16266:2006
Form:
powder
Suitability:
nonselective and differential for Pseudomonas spp.
Application(s):
agriculture
environmental
food and beverages
water monitoring, microbiology
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agency

according to ISO 16266:2006

Quality Segment

sterility

non-sterile

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 10 g/L , dipotassium hydrogen phosphate, 1.5 g/L , magnesium sulfate, 1.5 g/L , mixed peptone, 20 g/L

packaging

pack of 500 g

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbe id | metabolite detection: suitable, microbiological culture: suitable

final pH

7.2±0.2 (25 °C)

application(s)

agriculture
environmental
food and beverages
water monitoring, microbiology

suitability

nonselective and differential for Pseudomonas spp.

General description

King′s B Agar is a specialized microbiological medium designed to enhance the production of fluorescein while inhibiting the formation of pyocyanin. The medium contains mixed peptones that provide essential nitrogenous nutrients, carbon, sulfur, and trace elements. Glycerol serves as a carbon source, while dipotassium hydrogen phosphate acts as a buffering agent. Magnesium sulfate is included to activate fluorescein production. When used for culturing Pseudomonas aeruginosa, the colonies are surrounded by a yellow to greenish-yellow zone due to fluorescein production, which fluoresces under UV light. If pyocyanin is also synthesized, a bright green color is observed. The medium is particularly useful for differentiating Pseudomonas aeruginosa from other species like Pseudomonas fluorescens and Pseudomonas putida, as the latter do not fluoresce under UV light and grow poorly on MacConkey Agar.

Application

用于检测和计数水中的荧光细菌,特别是饮用水中的荧光假单胞菌。

Features and Benefits

NutriSelect® Basic is a powdered culture media offering
  • Quality control with growth promotion test according to industry standards
  • Quality assurance acc. to ISO 9001
  • Cost-efficient powdered media

Preparation Note

取33g溶于990ml蒸馏水中,并添加10ml甘油(货号49769)。121°C下高压蒸汽灭菌15分钟。
可选择添加CFC选择性补充剂(货号53477)制备选择性培养基。

Other Notes

多孔板应用
我们现提供两种类型的培养基:GranuCult®优质颗粒状培养基和NutriSelect®高性价比粉末状培养基,满足用户不同层次的需求。
商标后的品级称号basic(基础)、plus(加强)或prime(特级)代表产品的不同质控水平,从基础的质控到标准质控加强版到全面合规的特级质控水平。

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

This product will be discontinued in the near future; however, stock is still available. The recommended replacement product is 1.10991 - KING agar B (base) (Dansk Standard)


存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



历史批次信息供参考:

分析证书(COA)

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