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Merck
CN

69966

MRS肉汤

NutriSelect® Basic, powder, for Lactobacillus spp., for Bifidobacterium spp., pack of 500 g

别名:

乳酸杆菌肉汤（根据De Man，Rogosa和Sharpe的报道）

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关于此项目

NACRES:
NA.74
UNSPSC Code:
41171607
Manufacturer/tradename:
NutriSelect® Basic
Ph:
6.2±0.2
Form:
powder
Suitability:
Bifidobacterium spp., Lactobacillus spp.
Application(s):
agriculture
bioburden testing
environmental
food and beverages
microbiology

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sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

dipotassium hydrogen phosphate, 2 g/L , glucose, 20 g/L , magnesium sulfate monohydrate, 0.2 g/L , manganous sulfate tetrahydrate, 0.05 g/L , meat extract, 8 g/L , peptone, 10 g/L , sodium acetate, 5 g/L , triammonium citrate, 2 g/L , yeast extract, 4 g/L

packaging

pack of 500 g

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

pH

6.2±0.2

application(s)

agriculture
bioburden testing
environmental
food and beverages
microbiology

suitability

Bifidobacterium spp., Lactobacillus spp.

Quality Level

100

相关类别

General description

MRS 肉汤用于从所有类型的材料中富集、培养和分离所有种类的乳酸杆菌（根据 DeMan、Rogosa 和 Sharpe 的报道）。该培养基可用于从 MRS 琼脂中分离出的菌落的确认测试。MRS 培养基含有聚山梨醇酯（Tween 80）、醋酸盐、镁和锰，这些都是乳酸杆菌的已知的特殊生长因子，并且是丰富的营养基础。由于这些培养基显示出非常低的选择性，片球菌和明串珠菌以及其他继发细菌可能会在其中生长。大多数伴随的微生物群落可以被醋酸铊、山梨酸、醋酸、亚硝酸钠、放线菌素和多粘菌素抑制。这些物质可以以不同的浓度和组合使用，但不可避免地必须在有机体的选择性和回收率之间达成妥协。

Application

MRS 肉汤用于从所有类型的材料中富集、培养、分离所有种类的乳酸杆菌。

Preparation Note

将 51.25 g 溶解在 1 升蒸馏水中并加入 1 ml Tween® 80（货号 P8074）。加热煮沸至完全溶解培养基。装入容器并通过121°C高压灭菌15 分钟进行灭菌。在 35°C 下孵育培养物最多3天，或在30°C下最多孵育 5 天。如果可能，在富含二氧化碳的厌氧罐中孵育培养物。

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

Disclaimer

我们现提供两种类型的培养基：GranuCult®优质颗粒状培养基和NutriSelect®高性价比粉末状培养基，满足用户不同层次的需求。商标后的品级称号basic（基础）、plus（加强）或prime（特级）代表产品的不同质控水平，从基础的质控到标准质控加强版到全面合规的特级质控水平。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

监管及禁止进口产品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCN6889
BCCN6888
BCCN6109
BCCN6110
BCCN2039

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P Laloi et al.
Applied microbiology and biotechnology, 36(2), 196-204 (1991-11-01)
Whole cells of Lactobacillus delbrueckii subsp. bulgaricus CNRZ 397 were able to hydrolyse alpha- and beta-caseins. Irrespective of the growth medium used, milk or De Man-Rogosa-Sharpe (MRS) broth, identical patterns of alpha- and beta-casein hydrolytic products, respectively, were visualized by
L.C. Laleye et al.
Journal of Food Protection, 50, 1009-1009 (1987)
J.D. DeMan et al.
The Journal of Applied Bacteriology, 23, 130-130 (1960)
Sonja Blasche et al.
Nature microbiology, 6(2), 196-208 (2021-01-06)
Microbial communities often undergo intricate compositional changes yet also maintain stable coexistence of diverse species. The mechanisms underlying long-term coexistence remain unclear as system-wide studies have been largely limited to engineered communities, ex situ adapted cultures or synthetic assemblies. Here
Rupal Mistry et al.
Genetics, 206(2), 889-904 (2017-04-18)
Resident gut bacteria are constantly influencing the immune system, yet the role of the immune system in shaping microbiota composition during an organism's life span has remained unclear. Experiments in mice have been inconclusive due to differences in husbandry schemes

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