Merck
CN
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文件

安全信息

70138

Millipore

脑心浸液琼脂

suitable for microbiology, NutriSelect® Plus, For the cultivation of fastidious, pathogenic bacteria, yeasts, and molds

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别名:
BHI 琼脂
NACRES:
NA.74

质量水平

200

无菌性

non-sterile

产品线

BioChemika

形式

powder

保质期

limited shelf life, expiry date on the label

组成

agar, 15 g/L
brain extract, 7.8 g/L
dextrose, 2.0 g/L
disodium phosphate, 2.5 g/L
heart extract, 9.7 g/L
proteose peptone, 10.0 g/L
sodium chloride, 5.0 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | specific enzyme detection: suitable
microbiological culture: suitable

最终pH

7.4±0.2 (25 °C)

pH值（酸碱度）

7.2-7.6 (25 °C)

application(s)

clinical testing
environmental
food and beverages
life science and biopharma
water monitoring
microbiology

储存温度

10-30°C

适用性

Ajellomyces spp.
Blastomyces spp.
Enterococcus spp.
Erysipelothrix spp.
Haemophilus spp.
Pneumococcus spp.
nonselective for Candida spp.
nonselective for Clostridium spp.
nonselective for Escherichia coli
nonselective for Lactobacillus spp.
nonselective for Neisseria spp.
nonselective for Shigella spp.
nonselective for Staphylococcus spp.
nonselective for Streptococcus spp.
nonselective for bacteria (General Media)
nonselective for coliforms

相关类别

一般描述

Rosenow 通过将脑组织添加到葡萄糖琼脂中设计了原始培养基。这些培养基营养丰富步步高并且缓冲良好，可支持多种微生物的生长，例如链球菌、肺炎球菌、脑膜炎球菌等。加入腹水可以培养淋球菌。由于添加了10%脱纤维羊血，它可用于分离培养荚膜组织胞浆菌和其它病原真菌。该培养基用于选择性分离难养真菌（尤其是荚膜组织胞浆菌和芽生菌），通过添加以下推荐的成分混合物，几乎可以完全抑制伴生细菌和腐生酵母菌和霉菌的生长：每升 12 毫克青霉素和 40 微克链霉素； 每升 50 毫克氯霉素和 500 毫克放线菌酮； 每升 50 毫克庆大霉素和/或 50 毫克氯霉素。
当添加血液时，由于其葡萄糖含量，该培养基不太适合鉴定溶血形式。在处理荚膜组织胞浆菌时应格外小心，以避免其感染性孢子的传播。培养物应在封闭的过滤空气柜中检查。

应用

BHI 琼脂是一种通用培养基，用于从临床标本中初步分离致病性需氧菌，以及培养和维持食品、动物饲料、水和其他样品中的难养微生物。
添加到基础培养基中的脱纤维羊血为难养真菌微生物提供了必需的生长因子。

制备说明

取52 g溶于1L蒸馏水中。加热煮沸至完全溶解培养基。分装到试管或瓶中，并在121°C下高压蒸汽灭菌15分钟。

其他说明

选择溶血性葡萄球菌临床分离株对万古霉素的耐药性。

附注

我们现提供两种类型的培养基：GranuCult®优质颗粒状培养基和NutriSelect®高性价比粉末状培养基，满足用户不同层次的需求。
商标后的品级称号basic（基础）、plus（加强）或prime（特级）代表产品的不同质控水平，从基础的质控到标准质控加强版到全面合规的特级质控水平。

法律信息

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物来源培养基

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R S Schwalbe et al.
The Journal of infectious diseases, 161(1), 45-51 (1990-01-01)
Killing curves were used to characterize Staphylococcus haemolyticus isolates previously reported to contain subpopulations showing increased resistance to vancomycin. Results suggested that vancomycin and teicoplanin were ineffective at a concentration of 8 micrograms/ml and growth was seen between 24 and
Monica Gupta et al.
Nature communications, 6, 6379-6379 (2015-03-17)
Autophagy, activated by many stresses, plays a critical role in innate immune responses. Here we show that interferon regulatory factor 8 (IRF8) is required for the expression of autophagy-related genes in dendritic cells. Furthermore in macrophages, IRF8 is induced by
Bettina Schmid et al.
Biomedicines, 8(7) (2020-07-08)
Recently, the role of infection of the intervertebral disc (IVD) with Cutibacterium acnes (C. acnes) as a contributor to disc-related low back pain (LBP) has been discussed. The aim of this study was to investigate whether and how C. acnes
Marie-Paule Nawrot-Esposito et al.
Insects, 11(10) (2020-10-18)
Bioinsecticides made from the bacterium Bacillus thuringiensis (Bt) are the bestselling bioinsecticide worldwide. Among Bt bioinsecticides, those based on the strain Bt subsp. kurstaki (Btk) are widely used in farming to specifically control pest lepidopteran larvae. Although there is much
Juan Tur et al.
Cell reports, 32(8), 108079-108079 (2020-08-28)
Mitofusin 2 (Mfn2) plays a major role in mitochondrial fusion and in the maintenance of mitochondria-endoplasmic reticulum contact sites. Given that macrophages play a major role in inflammation, we studied the contribution of Mfn2 to the activity of these cells.

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