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85927

Millipore

HiCrome™ ECC 选择性培养基

NutriSelect® Plus, suitable for microbiology

别名:

ECC 选择性培养基 HiCrome™, 大肠杆菌-大肠菌选择性培养基 HiCrome™

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About This Item

MDL编号:
MFCD01634645
UNSPSC代码:
41171606
NACRES:
NA.85

无菌性

non-sterile

质量水平

200

表单

powder

保质期

limited shelf life, expiry date on the label

组成

agar, 10.0 g/L
casein enzymic hydrolysate, 3.3 g/L
chromogenic mixture, 0.43 g/L
disodium hydrogen phosphate, 1 g/L
peptone, special, 6.0 g/L
sodium chloride, 2 g/L
sodium dihydrogen phosphate, 0.6 g/L
sodium pyruvate, 1 g/L
sorbitol, 1 g/L
Tergitol™
7, 0.15 g/L
tryptophan, 1 g/L

制造商/商品名称

NutriSelect® Plus

最终pH

6.8±0.2 (25 °C)

应用

agriculture
bioburden testing
environmental
food and beverages
water monitoring
microbiology

适用性

selective and differential for Citrobacter spp.
selective and differential for Enterobacter spp.
selective and differential for Escherichia coli
selective and differential for Salmonella spp.
selective and differential for Shigella spp.
selective and differential for coliforms
selective and differential for enterobacteriaceae

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相关类别

应用

HiCrome™ ECC 选择性琼脂是一种选择性培养基，推荐用于对水和食物样品中的大肠杆菌和大肠菌群进行同时检测。该培养基中的成分甚至有助于亚致死性损伤的大肠菌迅速生长，Tergitol™ 可以抑制革兰氏阳性和某些革兰氏阴性细菌，而不影响大肠菌。显色混合物包含两种显色底物：Salmon-GAL 和 X-葡萄糖苷酸。大肠菌产生的 β-D-半乳糖苷酶可裂解 Salmon-GAL，使大肠菌菌落呈现鲜肉色至红色。大肠杆菌产生的 β-D-葡萄糖醛酸苷酶则可裂解 X-葡萄糖苷酸。由于 Salmon-GAL 和 X-葡萄糖苷酸均被裂解，因此大肠杆菌形成深蓝色到紫色的菌落。添加色氨酸可增进吲哚反应。为了确认大肠杆菌，可将一滴柯氏试剂（目录号 60983）滴在深蓝色到紫色的菌落上。形成樱桃红色则说明反应呈阳性。

制备说明

Suspend 26.5 g in 1 litre distilled water. Heat in a boiling water bath or in a free flowing steam, with stirring to dissolve the medium completely (approximately 35 minutes). Medium may show haziness, but it does not affect the performance. If desired to inhibit Pseudomonas and Aeromonas species add 5 mg Cefsulodin (Cat. No. 22126) for surface and pour plate method and 10 mg Cefsulodin for membrane filter technique. Mix well.

重悬

Suspend 26.5 g in 1 liter of distilled water. Heat in a boiling water bath or in a free flowing steam, with stirring to dissolve the medium completely (approximately 35 minutes). Medium may show haziness, but it does not affect the performance. If desired to inhibit Pseudomonas and Aeromonas species, add 5 mg of cefsulodin for surface and pour plate method and 10 mg of cefsulodin for membrane filter technique. Mix well.

附注

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

法律信息

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
TERGITOL is a trademark of The Dow Chemical Company ("Dow") or an affiliated company of Dow

象形图

Exclamation mark
GHS07

警示用语：

Warning

危险声明

H317

危险分类

Skin Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCN2022
BCCL3003
BCCH4374
BCCG1479
BCCB8207

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