861006
伊红 B
certified by the BSC, Dye content 90 %
别名:
4′,5′-二溴-2′,7′-二硝基荧光素 二钠盐, Saffrosine, 伊红猩红, 伊红蓝, 酸性红91
Agency
certified by the BSC
质量水平
表单
powder
组成
Dye content, 90%
技术
microbe id | staining: suitable
溶解性
H2O: 1 mg/mL, clear, red
λmax
395 nm
514 nm
应用
diagnostic assay manufacturing
hematology
histology
储存温度
room temp
SMILES字符串
[Na+].[Na+].[O-]C(=O)c1ccccc1C2=C3C=C(C(=O)C(Br)=C3Oc4c(Br)c([O-])c(cc24)[N+]([O-])=O)[N+]([O-])=O
InChI
1S/C20H8Br2N2O9.2Na/c21-14-16(25)11(23(29)30)5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(24(31)32)17(26)15(22)19(10)33-18(9)14;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2
InChI key
GYYTYUGGVBYJHE-UHFFFAOYSA-L
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应用
- 曙红B已用于从大鼠获得的肛门括约肌切片的组织学分析。
- 它已与Mayer′s苏木精一起用于胸骨三角肌切片的染色。
- 它已与苏木精一起用于从小鼠获得的不同组织切片的染色。
在Mayer′s Hemalum之后对胶原蛋白进行复染。与天青A一起用作细胞颗粒，细胞核和微生物的组织染色剂。用于垂体前叶细胞的差异染色。
生化/生理作用
曙红B是一种呫吨染料，也称为曙红蓝或赤藓红B。它用于Negri小体的Harris染色。曙红B还用于Mayer′s Hemalum中，作为胶原蛋白的复染剂。曙红B-天青A一起用于细胞颗粒、细胞核和微生物的染色。也可用于蛋白估计值。蛋白-曙红B复合物在536-544nm波长范围内吸收，蓝移的变化有助于确定蛋白的浓度。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
Restoration of anal sphincter function after myoblast cell therapy in incontinent rats.
Bisson A, et al.
Cell Transplantation, 24, 277-277 (2015)
Theory and Practice of Histological Techniques (2008)
A A Waheed et al.
Journal of biochemical and biophysical methods, 33(3), 187-196 (1996-12-30)
We describe a detailed procedure for estimating a wide range of proteins by the eosin B dye method. At acidic pH, eosin B binds to proteins and absorption of the protein-dye complex at 536-544 nm is proportional to the concentration
Syeda Sara Hassan et al.
Journal of hazardous materials, 190(1-3), 1030-1036 (2011-05-13)
We synthesized mefenamic acid (MA) derived gold nanoparticles (MA-AuNps) in aqueous solution (MA-Au sol). Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) of the sol at 1, 5, 15 and 60 min showed changes in size and shape of
Y M Cheng et al.
Neuroscience, 151(4), 1084-1098 (2008-01-19)
We examined the potential contribution of a voltage-gated proton conductance (gH+) to acid extrusion from cultured postnatal rat hippocampal neurons. In neurons loaded with Ca2+- and/or pH-sensitive fluorophores, transient exposures to 25-139.5 mM external K+ (K+o) or 20 microM veratridine
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