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安全信息

94448

Sigma-Aldrich

革兰氏结晶紫 溶液

for microscopy

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别名:
Basic violet 3; gentian violet; hexamethyl pararosaniline; methyl violet 10B
NACRES:
MA.02

等级

for microscopy

质量水平

200

产品线

BioChemika

形式

liquid

保质期

limited shelf life, expiry date on the label

technique(s)

microbe id | staining: suitable

颜色

purple to very dark purple

密度

0.981 g/mL at 20 °C

εmax

33.8 at 585-595 nm in water

适用性

suitable for microscopy

抗生素抗菌谱

Gram-negative bacteria
Gram-positive bacteria

application(s)

diagnostic assay manufacturing
hematology
histology

储存温度

room temp

相关类别

一般描述

Gram’s crystal violet solution, also known as gentian violet or hexamethyl pararosaniline, is a basic, cationic, acidotropic aminoarylmethane dye. It is a hexa-N-methylated triaminotriphenylmethane with a moderately sized lipophilic cation and a slightly nonplanar conjugated system. It is used in bacteriology as the primary stain in Gram staining to differentiate Gram-positive and Gram-negative bacteria. It is known to possess antibacterial and antifungal properties.

应用

  • Crystal violet is routinely used for Gram staining and its variants, staining amyloid, bacterial components, and vascular plant tissues.
  • It is used in polychrome staining of epoxy resin sections, viability staining of cultured neurons, and confocal optical sectioning to analyze meiotic structures.
  • It is also employed in the acridine orange-crystal violet staining of intracellular bacteria, microsporidian spores, and cytological smears.

生化/生理作用

The antimicrobial effects of crystal violet are caused by free radical generation and the formation of unionized dye-bacteria complexes. It also inhibits bacterial protein synthesis and cell wall formation and is especially effective against Gram-positive bacteria.

原理

Crystal violet is the primary stain in Gram staining. It infiltrates the peptidoglycan-rich cell wall of Gram-positive bacteria, and forms complexes with Gram’s iodine that are entrapped and retain the purple color even after decolorization. Thus, Gram-positive bacteria can be differentiated from Gram-negative bacteria whose peptidoglycan layer is considerably thinner and does not retain crystal violet.

警示用语：

Warning

危险分类

Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Flam. Liq. 3

储存分类代码

3 - Flammable liquids

WGK

WGK 2

闪点(°F)

93.2 °F

闪点(°C)

34 °C

个人防护装备

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

法规信息

危险化学品

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Conn's Biological Stains
Kiernan & Horobin
Conn?s Biological Stains (2002)
Mantas Šilkūnas et al.
The Journal of membrane biology, 249(5), 611-621 (2016-04-06)
In this study, the role of the cell plasma membrane as a barrier in the mechanism of the cytotoxicity of nitrogen-containing bisphosphonates and menadione was studied, and the possibility of increasing the efficiency of bisphosphonates and menadione (vitamin K
Oihana Gordobil et al.
International journal of biological macromolecules, 135, 353-361 (2019-05-28)
Kraft lignin is a polyphenolic compound generated as a by-product from the kraft pulping process in large quantities annually worldwide. In addition to its commercial availability, its structural features make it worth to be considered in the pharmaceutical area. The
Alexander M Maley et al.
Experimental dermatology, 22(12), 775-780 (2013-10-15)
Gentian violet (GV) has a long and varied history as a medicinal agent. Historically used as an antibacterial and antifungal, recent reports have shown its utility as an antitypranosomal, antiviral and anti-angiogenic agent. The objective of this article is to

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